摘要
探索不同添加量丹参药渣培养对平菇菌丝生长、子实体生物学性状、产量和体外抗氧化活性的影响。结果表明:以栽培基质总质量为基准,最优栽培基质配方为丹参药渣20%、棉籽壳60%、麦麸16%、石灰2%、石膏1%、白砂糖1%。在此条件下,平菇菌丝生长较快,生物学效率、子实体产量、蛋白质和多糖含量明显提高;与不添加丹参药渣组比,平菇黄酮和多酚含量分别提高23.21%和8.83%,对DPPH自由基和ABTS自由基的清除率分为72.68%和66.09%。经体外模拟消化后,多酚释放量为胃消化>肠消化>口腔消化,黄酮释放量和ABTS自由基清除率为肠消化>胃消化>口腔消化,DPPH自由基清除率为胃消化≈肠消化>口腔消化,且最优栽培基质配方种植的平菇黄酮、多酚释放量及体外抗氧化活性均有明显优势。
The effects of different addition amounts of Salvia miltiorrhiza residue cultivation on the growth of Pleurotus ostreatus,the biological characteristics of fruiting bodies,yield,and in vitro antioxidant ac-tivity were explored.The results showed that based on the total mass of the cultivation substrate,the opti-mal cultivation substrate formula was 20%Salvia miltiorrhiza residue,60%cottonseed hulls,16%wheat bran,2%lime,1%gypsum,and 1%white sugar.Under these conditions,the mycelium of Pleurotus ostreatus grows rapidly,and the biological efficiency,the yield of fruiting bodies,and the con-tents of protein and polysaccharides were significantly increased.Compared with the group without the addition of Salvia miltiorrhiza residue,the contents of flavonoids and polyphenols in Pleurotus ostreatus were increased by 23.21%and 8.83%,respectively,and the scavenging rates of DPPH free radicals and ABTS free radicals were 72.68%and 66.09%,respectively.After in vitro simulated digestion,the release amount of polyphenols was found to follow the order of gastric digestion>intestinal digestion>oral digestion,the release amount of flavonoids and the scavenging rate of ABTS free radicals were found to follow the order of intestinal digestion>gastric digestion>oral digestion,and the scavenging rate of DPPH free radicals was found to follow the order of gastric digestion~intestinal digestion>oral digestion.Moreover,the Pleurotus ostreatus cultivated with the optimal cultivation substrate formula was showed to have obvious advantages in the release amounts of flavonoids and polyphenols as well as the in vitro antioxidant activity.
作者
陈亚蓝
任双
杨玲玲
郝雨萌
刘佳依
朱静
CHEN Yalan;REN Shuang;YANG Lingling;HAO Yumeng;LIU Jiayi;ZHU Jing(Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China;Henan Dabie Mountain Characteristic Food Resources Comprehensive,Utilization Engineering Technology Research Center,Xinyang 464000,Henan,China)
出处
《粮食与油脂》
北大核心
2025年第9期102-108,共7页
Cereals & Oils
基金
河南省重点研发专项(231111312800)
河南省高校研究性教学改革与实践研究项目(2022SYJXLX119)
河南省高等学校重点科研项目计划(24B550017、24B180016)
河南省重点研发与推广专项(科技攻关)项目(242102110090、242102110144)
信阳农林学院科技创新团队(XNKJTD-001)。
关键词
丹参药渣
平菇
体外抗氧化活性
模拟消化
Salvia miltiorrhiza residue
Pleurotus ostreatus
in vitro antioxidant activity
simulated digestion
作者简介
陈亚蓝(1991-),女,硕士,讲师,研究方向为天然活性产物开发研究及食品加工;通信作者:朱静(1983-),女,博士,副教授,研究方向为食品生物技术。
