摘要
对已克隆化分离的一个β地贫基因,应用6种人工合成的寡核苷酸为内引物。以[α-^(35)S]dATP进行标记,用Sanger双脱氧末端终止法进行序列测定。结果表明,此例β珠蛋白基因的密码子41/42中缺失了4个核苷酸(TCTT)。
DNA sequence of βg-lobin gene from a patient with p-thalassemia major in Guangdong province was subcloned into Ml3mp 19 and analyzed by dideoxy-chain termination method of Sanger,using six 19mer oligonu-cleotides as internal primers and (α-^(35)S) dATP for labeling in synthetic mixture.A four-nucleotide deletion in codon 41 and 42 was found.This is the firstβ-thalassemia gene cloned and sequenced in China.
作者
周小燕
黄尚志
王涛
罗会元
王申五
杨善蓉
蔡若莲
Zhou Xiaoyan(Institute of Basic Medical Sciences,Academiae Medicinae Sinicae,Beijing)
出处
《中国医学科学院学报》
1988年第1期53-56,共4页
Acta Academiae Medicinae Sinicae
