摘要
为探究茄子(Solanum melongena L.)SmMYB 13基因在干旱胁迫中的功能,从茄子中克隆转录因子SmMYB 13,进行生物信息学分析并检测干旱胁迫下该基因的表达情况。采用花序浸染法在拟南芥(Arabidopsis thaliana)中异源过表达SmMYB 13基因,分析转基因植株的抗旱性和脱落酸(abscisic acid,ABA)相关基因的表达。结果表明,SmMYB 13基因全长777 bp,编码258个氨基酸,属于亲水性不稳定蛋白,无信号肽和跨膜结构,主要定位于细胞核和线粒体。系统进化树分析表明,SmMYB13蛋白与马铃薯和番茄MYB13蛋白的亲缘关系较近。qRT-PCR分析显示,SmMYB 13基因在茄子根、茎、叶、果皮、花和果肉中均有表达,在果皮中的表达水平最高。干旱胁迫下,与野生型拟南芥相比,SmMYB 13基因过表达拟南芥的叶片细胞膜通透性降低,不易被甲苯胺蓝(toluidine blue,TB)染色,失水率显著下降,超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和过氧化氢酶(catalase,CAT)活性显著升高,MDA含量降低,抗旱能力增强。ABA处理下,与野生型拟南芥相比,SmMYB 13基因过表达拟南芥的ABA负调节基因表达水平上调,正调节基因表达水平下调,对ABA的敏感性降低。上述结果说明,茄子SmMYB 13基因是干旱胁迫响应因子,在茄子干旱胁迫中起正调控作用。
To explore the function of the SmMYB 13 gene in eggplant(Solanum melongena L.)under drought stress,the transcription factor SmMYB 13 was cloned from eggplant,and bioinformatics analysis and expression detection under drought stress were conducted.The SmMYB 13 gene was heterologously overexpressed in Arabidopsis thaliana using the inflorescence infiltration method,and the drought resistance and the expression of abscisic acid(ABA)-related genes in transgenic plants were analyzed.The results showed that the full length of the SmMYB 13 gene was 777 bp,encoding 258 amino acids.It was a hydrophilic unstable protein without signal peptide and transmembrane structure,and was mainly located in the nucleus and mitochondria.Phylogenetic tree analysis revealed that the SmMYB13 protein was closely related to MYB13 proteins from Solanum tuberosum L.(potato)and Solanum lycopersicum L.(tomato).qRT-PCR analysis showed that the SmMYB 13 gene was expressed in the root,stem,leaf,pericarp,flower and flesh of eggplant,with the highest expression level in the pericarp.Under drought stress,compared with wild-type A.thaliana,SmMYB 13-overexpression A.thaliana exhibited reduced cell membrane permeability in leaf,was less stained by toluidine blue(TB),the water loss rate significantly decreased,while the activities of antioxidant enzymes,including superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT),were significantly enhanced,malondialdehyde(MDA)content decreased,and drought resistance improved.Under ABA treatment,compared with wild-type A.thaliana,SmMYB 13-overexpression A.thaliana showed upregulated expression of ABA-negative regulator genes and downregulated expression of ABA-positive regulator genes,resulting in reduced sensitivity to ABA.The above results indicated that the SmMYB 13 gene in eggplant was a drought stress response factor and played a positive regulatory role in drought stress.
作者
李宇静
黄倩茹
张爱冬
吴雪霞
朱栋幸
肖凯
LI Yujing;HUANG Qianru;ZHANG Aidong;WU Xuexia;ZHU Dongxing;XIAO Kai(College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;Protected Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Agriculture and Rural Service Center,Zhangyan Town,Jinshan District,Shanghai,Shanghai 201514,China)
出处
《浙江农业学报》
北大核心
2025年第8期1666-1679,共14页
Acta Agriculturae Zhejiangensis
基金
国家大宗蔬菜产业技术体系上海综合试验站项目(CARS-23-G41)
上海市农业科学院卓越团队项目(沪农科卓[2022]019)。
关键词
茄子
转录因子
SmMYB
13基因
干旱胁迫
拟南芥
Solanum melongena L.
transcription factor
SmMYB 13 gene
drought stress
Arabidopsis thaliana
作者简介
李宇静(2000-),女,河南新乡人,硕士,主要从事茄子遗传育种研究。E-mail:zyllyj@163.com;通信作者:肖凯,E-mali:15562791500@163.com。
