摘要
2-甲基异莰醇(2-methylisoborneol,2-MIB)是一种水中常见的异味物质。土塘是鳗鲡养殖的主要模式之一,残饵及粪便的累积易造成水体富营养化,加速有害藻类和细菌大量繁殖,进而导致养殖水体土腥味物质(如2-MIB)的积累并影响鱼类的生长和品质。为探究2-MIB胁迫对日本鳗鲡(Anguilla japonica)的影响机制,将日本鳗鲡暴露在不同浓度(10和20μg/L)的2-MIB中96 h,测定鱼体肌肉中2-MIB的含量,并对鳃组织进行组织学和转录组测序分析。结果表明,肌肉中2-MIB含量随暴露浓度的增加而增加,2-MIB积累量从(0.83±0.10)μg/kg增加至(13.42±0.31)μg/kg。组织学观察表明,10和20μg/L浓度的2-MIB分别引起日本鳗鲡鳃组织空泡化和炎症细胞的聚集。转录组分析表明在10μg/L暴露浓度下有38个基因显著上调,73个基因显著下调;在20μg/L暴露浓度下有143个基因显著上调,79个基因显著下调。KEGG通路富集筛选出与免疫应激和炎症反应相关的通路如横纹肌细胞发育通路、MAPK通路等。2-MIB暴露下会造成日本鳗鲡卟啉代谢紊乱,产生炎症反应,通过信号传导调节氧化应激。研究结果揭示了日本鳗鲡在2-MIB胁迫下鳃组织的分子调控机制以及2-MIB的潜在毒性,为鳗鲡养殖管理及土腥味防控提供了重要参考。
2-methylisoborneol(2-MIB)is a common off-flavor substance in water.Earthen ponds are one of the main modes of eel farming.However,the accumulation of excessive feed and animal feces leads to water eutrophication,accelerates the proliferation of harmful algae,and causes the accumulation of earthy odor substances(such as 2-MIB)in the aquaculture water,which in turn affects the growth and quality of eels.To explore the mechanism of 2-MIB stress on Japanese eels(Anguilla japonica),they were exposed to 2-MIB in different concentrations(10 and 20μg/L)of for 96 hours.The content of 2-MIB in fish muscle was determined,and histological analysis and transcriptome sequencing of gill tissues were conducted.Results show that the 2-MIB content in muscle increased with increasing exposure concentration,and 2-MIB accumulation increased from(0.83±0.10)to(13.42±0.31)μg/kg.Histological observations indicated that 2-MIB could cause vacuolation and the aggregation of inflammatory cells in the gill tissues.Transcriptome analysis revealed that at 10μg/L in 2-MIB concentration,38 genes were significantly upregulated and 73 genes were significantly downregulated;at 20μg/L,143 genes were significantly upregulated and 79 genes were significantly downregulated.KEGG pathway enrichment analysis screened out pathways related to immune stress and inflammatory responses,such as the skeletal muscle cell development pathway and the MAPK pathway.In addition,exposure to 2-MIB caused porphyrin metabolism disorders and inflammatory responses in the eels,and regulated oxidative stress through signal transduction.This study revealed the molecular regulatory mechanism of gill tissues of Japanese eels under 2-MIB stress and the potential toxicity of 2-MIB,providing an important reference for eel farming management and the prevention and control of earthy odor.
作者
沈紫倩
韩焕
靳锡辰
谢斌
曾祥标
倪国彬
周捷
陈桃英
李慷
刘利平
SHEN Zi-Qian;HAN Huan;JIN Xi-Chen;XIE Bin;ZENG Xiang-Biao;NI Guo-Bin;ZHOU Jie;CHEN Tao-Ying;LI Kang;LIU Li-Ping(China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology(Shanghai),Shanghai Ocean University,Shanghai 201306,China;Center for Ecological Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Chongming District Aquatic Technology Promotion Station,Shanghai 202150,China)
出处
《海洋与湖沼》
北大核心
2025年第4期1002-1011,共10页
Oceanologia Et Limnologia Sinica
基金
上海市崇明区农业科创项目,2022CNKC-01-06号
国家自然科学基金项目,32072994号。
作者简介
沈紫倩,硕士研究生,E-mail:yvonneshen97@163.com;韩焕,博士研究生,E-mail:huanhan86@foxmail.com,与第一作者同等贡献;通信作者:李慷,硕士生导师,副教授,E-mail:kli@shou.edu.cn;通信作者:刘利平,博士生导师,教授,E-mail:lp-liu@shou.edu.cn。
