摘要
试验旨在探究蒲公英提取物对产蛋后期蛋鸡生产性能、蛋品质、免疫指标、血清及肠道炎症相关因子基因表达的影响。将600只60周龄京粉6号蛋鸡随机分成4组,每组10个重复,每个重复15只蛋鸡。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加120、240和480 mg/kg蒲公英提取物。试验期70 d,包括7 d预试和63 d正试。结果显示:480 mg/kg蒲公英提取物显著提高产蛋后期蛋鸡平均蛋重(P<0.05)和产蛋率(P<0.05),呈一次线性关系(P<0.05);480 mg/kg蒲公英提取物显著降低产蛋后期蛋鸡料蛋比(P<0.05),呈一次线性关系(P=0.044);240和480 mg/kg蒲公英提取物显著提高蛋壳强度(P<0.05),呈一次线性关系(P=0.047);480 mg/kg蒲公英提取物显著提高哈氏单位(P<0.05),呈一次线性关系(P=0.025);蒲公英提取物显著提高蛋鸡血清免疫球蛋白A含量(P<0.05),呈一次线性关系(P=0.031)和二次曲线关系(P=0.013),480 mg/kg蒲公英提取物显著提高免疫球蛋白G含量(P<0.05),呈一次线性关系(P=0.043);480 mg/kg蒲公英提取物显著降低血清肿瘤坏死因子-α含量(P<0.05),呈一次线性关系(P=0.047),240和480 mg/kg蒲公英提取物显著降低白细胞介素-1β和内毒素含量(P<0.05),240和480 mg/kg蒲公英提取物显著降低蛋鸡血清D-乳酸含量(P<0.05);240和480 mg/kg蒲公英提取物显著提高回肠分泌型免疫球蛋白A含量(P<0.05),呈一次线性关系(P=0.018)和二次曲线关系(P=0.032),480 mg/kg蒲公英提取物显著降低肿瘤坏死因子-α含量(P<0.05),呈一次线性关系(P=0.032),480 mg/kg蒲公英提取物显著提高γ-干扰素含量(P<0.05),呈一次线性关系(P=0.041);480 mg/kg蒲公英提取物显著降低IL-1β、TLR4 mRNA表达水平(P<0.05),呈一次线性关系(P<0.05)。综合产蛋率、料蛋比、哈氏单位、血清免疫球蛋白A和G含量、血清D-乳酸含量、肠道分泌型免疫球蛋白A含量,产蛋后期蛋鸡饲粮蒲公英提取物适宜添加量为314.14~446.65 mg/kg。
The experiment aimed to explore the effects of dandelion extract on production performance,egg quality,immune indexes,gene expression of serum and intestinal inflammation-related factors of laying hens in late laying period.A total of 60060-week-age Jingfen No.6 laying hens were randomly divided into 4 groups with 10 replicates in each group and 15 hens in each replicate.The control group was fed a basal diet,while the experimental group was fed a basal diet supplemented with 120,240 and 480 mg/kg dandelion extract,respectively.The pre-test period was 7 days,and the formal test period was 63 days.The results showed that compared with the control group,480 mg/kg dandelion extract significantly increased the average egg weight(P<0.05)and laying rate(P<0.05)of laying hens in late laying period,showing a linear relationship(P<0.05);480 mg/kg dandelion extract significantly reduced the feed egg ratio of laying hens in late laying period(P<0.05),showing a linear relationship(P=0.044);240 and 480 mg/kg dandelion extract significantly increased eggshell strength(P<0.05),showing a linear relationship(P=0.047);480 mg/kg dandelion extract significantly increased the Haugh unit(P<0.05),showing a linear relationship(P=0.025);Dandelion extract significantly increased the serum immunoglobulin A content in laying hens(P<0.05),showing a linear relationship(P=0.031)and a quadratic curve relationship(P=0.013).480 mg/kg dandelion extract significantly increased the immunoglobulin G content(P<0.05),showing a linear relationship(P=0.043);480 mg/kg dandelion extract significantly reduced serum tumor necrosis factor-αlevels(P<0.05),showing a linear relationship(P=0.047).240 and 480 mg/kg dandelion extract significantly reduced interleukin-1βand endotoxin levels(P<0.05),while 240 and 480 mg/kg dandelion extract significantly reduced serum D-lactate levels in laying hens(P<0.05);240 and 480 mg/kg dandelion extract significantly increased the content of secretory immunoglobulin A in the ileum(P<0.05),showing a linear relationship(P=0.018)and a quadratic relationship(P=0.032).480 mg/kg dandelion extract significantly reduced the content of tumor necrosis factor-α(P<0.05),showing a linear relationship(P=0.032).480 mg/kg dandelion extract significantly increased the content of interferon-γ(P<0.05),showing a linear relationship(P=0.041);480 mg/kg dandelion extract significantly reduced the mRNA expression levels of IL-1βand TLR4(P<0.05),showing a linear relationship(P<0.05).Based on the comprehensive egg production rate,feed to egg ratio,Haugh unit,serum immunoglobulin A and G content,serum D-lactate content and intestinal secreted immunoglobulin A content,the appropriate amount of dandelion extract added to the feed for laying hens in late laying period was 314.14 to 446.65 mg/kg.
作者
张孝清
ZHANG Xiaoqing(Shuozhou Vocational Technical College,Shuozhou,Shanxi 036002)
出处
《中国家禽》
北大核心
2025年第9期38-44,共7页
China Poultry
基金
山西省职业教育教学改革与实践研究项目(202303063)。
关键词
蒲公英提取物
蛋鸡
蛋品质
生产性能
dandelion extract
layer
egg quality
production performance
作者简介
张孝清(1981-),男,硕士,副教授,主要从事中草药饲料添加剂研究,E-mail:1149032064@qq.com。
