摘要
目的探讨免疫组织化学(immunohistochemistry,IHC)染色与聚合酶链反应-毛细管电泳法(polymerase chain reaction-capillary electrophoresis,PCR-CE)检测微卫星不稳定状态的一致性和可靠性。方法收集行活检或手术切除的735例实体瘤患者的临床资料,采用IHC和PCR-CE法对组织样本进行微卫星状态检测。结果在344例结直肠癌(colorectal carcinoma,CRC)中,IHC结果显示:290例(84.3%)错配修复功能正常(proficient mismatch repair,pMMR),54例(15.7%)错配修复功能缺失(deficient mismatch repair,dMMR);PCR-CE检测结果显示:53例(15.4%)患者高度微卫星不稳定(high microsatellite instability,MSI-H),291例(84.6%)患者微卫星稳定(microsatellite stability,MSS)。IHC和PCR-CE检测结果的一致性分析显示Kappa值为0.923(P<0.001)。在168例子宫内膜癌(endometrial carcinoma,EC)中,IHC结果显示:42例(25.0%)dMMR,126例(75.0%)pMMR;PCR-CE检测结果显示:39例(23.2%)为MSI-H,129例(76.8%)为MSS。两种方法检测结果一致性分析显示Kappa值为0.854(P<0.001)。在165例胃癌中,IHC结果显示:有22例(13.3%)dMMR,143例(86.7%)pMMR;PCR-CE检测结果显示:22例(13.3%)为MSI-H,143例(86.7%)为MSS/微卫星低度不稳定(low microsatellite instability,MSI-L)。IHC和PCR-CE检测结果的一致性分析显示Kappa值为0.843(P<0.001)。58例头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中,IHC结果显示:有3例(5.2%)dMMR,55例(94.8%)pMMR;PCR-CE检测结果显示:有3例(5.2%)为MSI-H,55例(94.8%)为MSS。两种方法检测一致性分析显示Kappa值为1.000(P<0.001)。结论IHC和PCR-CE在检测CRC、EC、胃癌和HNSCC患者的MSI状态时具有较高的一致性和可靠性,联合两者应用有助于提高实体瘤患者MSI检测的准确率。
Purpose To investigate the consistency and reliability of immunohistochemistry(IHC)staining and polymerase chain reaction-capillary electrophoresis(PCR-CE)in the detection of microsatellite instability.Methods The clinical data of 735 patients with solid tumors who underwent biopsy or surgical resection were collected,and the microsatellite status of tissue samples was detected by IHC and PCR-CE.Results Among the 344 cases of colorectal cancer(CRC),IHC results showed that 290 cases(84.3%)had proficient mismatch repair(pMMR),and 54 cases(15.7%)had deficient mismatch repair(dMMR).The results of PCR-CE showed that 53 patients(15.4%)had high microsatellite instability(MSI-H),and 291 patients(84.6%)had microsatellite stable(MSS).The consistency analysis of IHC and PCR-CE results showed that the Kappa value was 0.923(P<0.001).Among the 168 cases of endometrial cancer(EC),IHC results showed that 42 cases(25.0%)had dMMR and 126 cases(75.0%)had pMMR;The results of PCR-CE showed that 39 cases(23.2%)had MSI-H and 129 cases(76.8%)had MSS.The consistency analysis of the two methods showed that the Kappa value was 0.854(P<0.001).Among the 165 cases of gastric cancer,IHC results showed that 22(13.3%)had dMMR and 143(86.7%)had pMMR;The results of PCR-CE showed that 22 cases(13.3%)had MSI-H and 143 cases(86.7%)had MSS/low microsatellite instability(MSI-L).The consistency analysis of IHC and PCR-CE results showed that the Kappa value was 0.843(P<0.001).Among the 58 cases of head and neck squamous cell carcinoma(HNSCC),IHC results showed that 3 cases(5.2%)had dMMR and 55 cases(94.8%)had pMMR;The results of PCR-CE showed that 3 cases(5.2%)had MSI-H and 55 cases(94.8%)had MSS.The consistency analysis of the two methods showed that the Kappa value was 1.000(P<0.001).Conclusion IHC and PCR-CE have high consistency and reliability in detecting MSI status in patients with CRC,EC,gastric cancer and HNSCC,and their combined application is helpful to improve the accuracy of MSI detection in patients with solid tumors.
作者
郭颖
程亚林
赵香宇
杨鑫鑫
孟宏学
Guo Ying;Cheng Yalin;Zhao Xiangyu;Yang Xinxin;Meng Hongxue(Department of Pathology,Harbin Medical University Cancer Hospital,Harbin 150081,China;Precision Medicine Center,Harbin Medical University Cancer Hospital,Harbin 150081,China)
出处
《临床与实验病理学杂志》
北大核心
2025年第8期1050-1056,共7页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(82072985)
北京精鉴病理学发展基金(JJTS2020-002)。
关键词
微卫星不稳定性
免疫组织化学
聚合酶链反应-毛细管电泳法
microsatellite instability
immunohistochemistry
polymerase chain reaction-capillary electrophoresis
作者简介
通讯作者:孟宏学,E-mail:menghongxue15@163.com。
