摘要
目的:通过网络药理学探讨中药处方柴胡疏肝散对癫痫合并抑郁的治疗前景。方法:通过中药系统药理学数据库与分析平台检索柴胡疏肝散有效成分及其对应靶点,在GeneCards、OMIM、PharmGkb、TTD及DrugBank数据库检索癫痫和抑郁相关靶点,与柴胡疏肝散作用靶点取交集。通过STRING数据库完成交集靶点的蛋白质–蛋白质相互作用网络的构建,利用Cytoscape预测其核心靶点。通过R软件对柴胡疏肝散治疗癫痫合并抑郁的共同靶点进行基因本体论(GO)功能分析及京都基因与基因组百科全书(KEGG)信号通路富集分析,最后利用Amdock软件进行分子对接,验证核心成分与核心靶点的结合能力。结果:共筛选出柴胡疏肝散活性成分259个,其主要活性成分为槲皮素、川陈皮素、木犀草素等;共得到癫痫、抑郁交集靶点4366个,经jvenn平台分析得到药物–疾病交集靶点190个,度值居前11位的靶点为肿瘤蛋白p53(Tumor Protein p53,TP53)、肿瘤坏死因子(Tumor Necrosis Factor,TNF)、信号转导和转录激活因子(Signal Transducer and Activator of Transcription,STAT)3、白细胞介素(Interleukin,IL)–6、IL–1β、B淋巴细胞瘤2基因(B–cell Lymphoma–2,BcL2)、丝氨酸/苏氨酸蛋白激酶1(Akt Serine/Threonine Protein Kinase 1,AKT1)、原癌基因FOS(FOS)、低氧诱导因子(Hypoxia Inducible Factor,HIF)–1A、STAT1、表皮生长因子受体(Epidermal Growth Factor Receptor,EGFR)。GO分析得到2 893个条目,KEGG分析得到信号通路196个,主要涉及磷脂酰肌醇3激酶(Phosphatidylinositol 3–kinase,PI3K)–蛋白激酶B(Akt)信号通路、IL–17信号通路、TNF信号通路等。分子对接结果表明柴胡疏肝散药物主要活性成分与癫痫合并抑郁核心靶点有较好的结合活性。结论:柴胡疏肝散通过抑制炎性反应、氧化应激反应及调节神经递质的释放等治疗癫痫合并抑郁。
Objective:To explore the application prospect of TCM prescription Chaihu Shugan San(柴胡疏肝散)in the treatment of epilepsy and depression co-morbidity through network pharmacology and molecular docking.Methods:The active ingredients and corresponding targets of Chaihu Shugan San were screened from TCMSP,and the targets related to epilepsy and depression were searched in GeneCards,OMIM,PharmGkb,TTD and DrugBank databases and intersected with the targets of Chaihu Shugan San.The protein-protein interaction network of the intersected targets was constructed by STRING,and the core targets were predicted by Cytoscape.The gene ontology(GO)functional analysis and Kyoto encyclopedia of genes and genomes(KEGG)signaling pathway enrichment analysis of the common targets of Chaihu Shugan San on epilepsy and depression co-morbidity were carried out by the R software.Finally,Amdock software was used to verify the binding ability of core active ingredients and core targets.Results:A total of 259 active components were screened out from Chaihu Shugan San,and the main active components were quercetin,nobiletin,luteolin,etc.There were 4366 intersection targets of epilepsy and depression.A total of 190 drug-disease intersection targets were obtained by the jvenn platform.Ranked by degree value,the top 11 targets were TP53,TNF,STAT3,IL-6,IL-1β,BcL-2,AKT1,FOS,HIF-1A,STAT1,and EGFR.A total of 2893 entries were obtained from GO analysis,and 196 signaling pathways were obtained from KEGG analysis,mainly involving PI3K-Akt signaling pathway,IL-17 signaling pathway,TNF signaling pathway,etc.The molecular docking results showed that the active ingredients of Chaihu Shugan San had better binding activity with the core targets of epilepsy and depression co-morbidity.Conclusion:Chaihu Shugan San may be able to treat epilepsy and depression co-morbidity by inhibiting inflammation and oxidative stress,and regulating the release of neurotransmitters.
出处
《中医临床研究》
2025年第14期106-114,共9页
Clinical Journal Of Chinese Medicine
基金
广西壮族自治区中医药管理局自筹经费科研课题(GZZC2020078)
广西中医药适宜技术开发与推广项目(GZSY21-39)。
关键词
癫痫
抑郁
柴胡疏肝散
网络药理学
分子对接
Epilepsy
Depression
Chaihu Shugan San
Network pharmacology
Molecular docking
作者简介
覃丽泉(1997-),女,广西河池人,2023级硕士研究生,研究方向为中西医结合神经内科疾病的防治与研究。;通讯作者:蒋媛静(1981-),广西桂林人,博士,副主任医师,研究方向为中西医结合神经内科疾病的防治与研究。
