摘要
目的:建立一种同时测定藏紫草中5种活性成分含量的方法。方法:采用Agilent SB-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.05%甲酸水(B)为流动相,检测波长510 nm,进样量为10μL,流速为1 mL/min,柱温30℃;采用聚类分析、主成分分析对不同产地藏紫草进行质量评价。结果:不同产地藏紫草5种活性成分含量差异较大,左旋紫草素含量为0.0027~0.0414 mg/g,乙酰紫草素含量为0.0158~1.1570 mg/g,去氧紫草素含量为0.0065~0.0544 mg/g,异丁酰紫草素含量为0.0477~0.5499 mg/g,β-β’-二甲基丙稀酰阿卡宁含量为0.0341~0.5011 mg/g。聚类分析将15批藏紫草样品分为3类。通过主成分分析对藏紫草质量进行综合评价,西藏山南、林芝产地药材质量较佳。结论:本研究建立了同时测定藏紫草5种活性成分的HPLC方法,结合聚类分析、主成分分析可用于藏紫草药材的质量评价。
Objective:To establish a method for simultaneous determination of five active components in Radix Onosmatis.Methods:High-performance liquid chromatography(HPLC)was performed on an Agilent SB-C18 column(4.6 mm×250 mm,5μm)with the mobile phase of acetonitrile(A)-0.05%formic acid solution(B)at a detection wavelength of 510 nm,injective volume at 10μL,a flow rate of 1 mL/min,and a column temperature of 30℃.The cluster analysis and the principal component analysis were used to perform quality assessment of Radix Onosmatis from different producing areas.Results:There were significant differences in the content of five active components between Radix Onosmatis from different producing areas,with a content of 0.0027-0.0414 mg/g for shikonin,0.0158-1.1570 mg/g for acetylshikonin,0.0065-0.0544 mg/g for deoxyshikonin,0.0477-0.5499 mg/g for isobutyrylshikonin,and 0.0341-0.5011 mg/g forβ-β’-dimethylacrylshikonin.The 15 batches of Radix Onosmatis were classified into three categories based on the cluster analysis.The principal component analysis was used for the comprehensive quality assessment of Radix Onosmatis,and the results showed that Radix Onosmatis from Shannan and Lingzhi of Xizang had a relatively good quality.Conclusion:This study establishes an HPLC method for simultaneous determination of five active components in Radix Onosmatis,and it can be used for the quality assessment of Radix Onosmatis in combination with the cluster analysis and the principal component analysis.
作者
谢景
唐雪阳
钟灿
张水寒
达瓦桑珠
王勇庆
XIE Jing;TANG Xueyang;ZHONG Can;ZHANG Shuihan;DAWA Sangzhu;WANG Yongqing(Institute of Traditional Chinese Medicine Resources,Hunan Academy of Chinese Medicine,Changsha 410013,Hunan,China;Shannan Tibetan Medicine Hospital of Xizang Autonomous Region,Shannan 856004,Xizang,China)
出处
《湖南中医杂志》
2025年第7期138-143,共6页
Hunan Journal of Traditional Chinese Medicine
基金
中央本级重大增减支项目名贵中药资源可持续利用能力建设项目(2060302)
西藏自治区藏医药管理局局级科技计划项目(JJKT2021014)。
关键词
藏紫草
质量评价
HPLC法
聚类分析
主成分分析
Radix Onosmatis
quality assessment
high-performance liquid chromatography
cluster analysis
principal component analysis
作者简介
第一作者:谢景,男,中药学博士,副研究员,研究方向:中药材规范化生产及其质量影响因素研究;通信作者:王勇庆,男,中药学硕士,副研究员,研究方向:中药资源及其鉴定研究,E-mail:wangyongqing00@126.com。
