摘要
保守寡聚高尔基体(conserved oligomeric Golgi,COG)复合物作为定位于高尔基体膜表面的栓系复合物,分为叶A(COG1-4)和叶B(COG5-8),通过COG1与COG8的动态连接形成完整的异八聚体结构。COG复合物通过与Rab GTP酶、SNARE蛋白及高尔基体CCT蛋白协同作用,参与COPⅠ囊泡逆行转运、COPⅡ囊泡顺行转运以及自噬等囊泡转运过程。COG复合物功能机制涵盖两大模型:其一为“拆卸组装模型”,即叶A锚定高尔基体膜,B叶结合囊泡膜,通过亚复合物动态组装拉近囊泡与靶膜距离;其二为“坞站装配模型”,完整COG复合物与SNARE、Rab等分子协同形成稳定对接平台,增强SNARE复合物稳定,促进膜融合效率。此外,COG复合体还参与巨自噬、Cvt通路和过氧化物酶体自噬在内的多种自噬途径。COG复合物的功能缺陷与Ⅱ型先天性糖基化障碍(congenital disorders of glycosylation,CDG-Ⅱ)密切相关,突变可引发糖基化紊乱,并成为相关疾病潜在的治疗靶点。本文通过COG复合物介导不同囊泡转运类型,介绍多个物种中COG复合物的调控机制,并总结该复合物在囊泡转运中协同作用的因子。
As a tethering complex localized on the Golgi membrane,the conserved oligomeric Golgi(COG)complex is divided into lobes A(COG1-4)and B(COG5-8)and forms a complete hetero-octamer structure through the dynamic linkage of COG1 to COG8.Through coordinated interactions with Rab GTPases,SNARE proteins,and Golgi-associated coiled-coil tethers(CCTs),the COG complex participates in coatomer complex I(COPⅠ)-mediated retrograde transport,coatomer complex II(COPⅡ)-mediated anterograde transport,and various autophagic processes.The functional mechanism of the COG complex includes two models.First,the"disassembly and assembly model",in which lobe A anchors the Golgi membrane,while lobe B binds to the vesicle membrane,and both lobes assemble into the complete COG complex to narrow the distance between vesicle and target membranes.Second,the"docking station assembly model",in which COG complexes cooperate with SNARE,Rab and other molecules to form a stable docking platform,to enhance the stability of SNARE complexes and promote membrane fusion efficiency.Additionally,the COG complex is involved in macroautophagy,the vacuole targeting(Cvt)pathway,and pexophagy.In this review,we introduce the regulatory mechanism of the COG complex in several species,and summarize the factors that have synergetic effects with the COG complex in vesicle transport.
作者
宋嘉宁
张浩林
王娟
SONG Jia-Ning;ZHANG Hao-Lin;WANG Juan(College of Chemistry and Life Science Department of Biology,Beijing University of Technology,Beijing 100124,China)
出处
《中国生物化学与分子生物学报》
北大核心
2025年第7期947-955,共9页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金项目(No.31970044)资助。
关键词
保守寡聚高尔基体
囊泡转运
细胞自噬
conserved oligomeric Golgi(COG)
vesicle trafficking
autophagy
作者简介
通讯作者:王娟,Tel:13581954897,E⁃mail:juanwang@bjut.edu.cn;共同第一作者:宋嘉宁;共同第一作者:张浩林。
