摘要
【目的】针对短程反硝化-厌氧氨氧化(PD-A)耦合工艺中厌氧氨氧化菌(AnAOB)持留率低的技术瓶颈,研究提出基于海藻酸钠(SA)-沸石复合载体的凝胶球固定化策略,旨在通过微环境调控与生物屏障协同作用强化AnAOB持留能力,突破传统悬浮污泥系统对复杂水质[如低碳氮比(C/N)、酸性条件]的适应性限制。【方法】通过批式试验系统评估了C/N=3.0~4.5及pH值为5.5~8.5条件下,对系统脱氮效能的调控影响。【结果】固定化AnAOB污泥的最大比活性达0.38 g N/(g VSS·d)。凝胶球可显著增强AnAOB对酸性环境(pH值≤6.5)的耐受性,而碱性条件(pH值=8.5)更利于短程反硝化过程中亚硝酸盐的快速积累及总氮(TN)的高效去除。最优C/N为4.0,当C/N<3.5或>4.5时,反硝化碳源竞争导致TN去除率下降。连续流试验证实,固定化系统运行30 d后仍维持84.4%的TN去除率,较未包埋系统提升20.3个百分点,表明凝胶球通过微环境调控与生物屏障作用有效强化了AnAOB持留能力。【结论】研究创新性地构建了兼具机械强度与传质效率的SA-沸石复合载体,通过包埋材料分子调控和功能菌群固定,为PD-A工艺处理复杂水质提供了理论依据与技术支撑。
[Objective]Aiming to address the technical bottleneck of low retention rate for anaerobic ammonia-oxidizing bacteria(AnAOB)in the coupled partial denitrification-anaerobic ammonia oxidation(PD-A)process,this study proposes an immobilization strategy using sodium alginate(SA)-zeolite composite gel spheres.The strategy synergistically enhances AnAOB retention through microenvironment regulation and biological barrier effects,overcoming the limitations of traditional suspended sludge systems in adapting to complex water conditions(e.g.,low C/N ratios and acidic environments).[Methods]The effects of carbon to nitrogen ratio(C/N=3.0-4.5)and pH value(5.5-8.5)on the modulation of systemic denitrification efficacy were evaluated by batch experimental system.[Results]The maximum specific activity of immobilized AnAOB sludge reached 0.38 g N/(g VSS·d).The result showed that gel spheres significantly enhanced the tolerance of AnAOB to the acidic environment(pH value≤6.5),while the alkaline condition(pH value=8.5)was more favorable for the rapid accumulation of nitrite and efficient TN removal during shortterm denitrification.The optimal C/N was 4.0,and the competition for denitrifying carbon sources led to a decrease in TN removal when C/N<3.5 or>4.5.Continuous flow experiments confirmed that the immobilized system maintained 84.4%TN removal after 30 days of operation,which was 20.3 percent point higher than that of the unembedded system,indicating that the gel spheres effectively strengthened the AnAOB retention capacity through microenvironmental regulation and biological barrier effects.[Conclusion]In this study,an innovative SA-zeolite composite carrier with dual functionalities of mechanical strength and mass transfer efficiency is constructed.The proposed strategy provides a theoretical basis and technical support for treating complex water quality via the PD-A process,achieved through molecular-level regulation of embedded materials and immobilization of functional bacterial consortia.
作者
孔海霞
KONG Haixia(Xi'an Branch,North China Municipal Engineering Design&Research Institute Co.,Ltd.,Xi'an 710018,China)
出处
《净水技术》
2025年第8期85-92,128,共9页
Water Purification Technology
基金
陕西省重点产业链项目(2022ZDLSF06-05)。
关键词
厌氧氨氧化
短程反硝化
固定化技术
凝胶载体
脱氮效能
anaerobic ammonia oxidation(Anammox)
partial denitrification
immobilization technology
gel carriers
nitrogen removal efficiency
作者简介
通信作者:孔海霞(1982-),女,高级工程师,主要从事污水、污泥处理与资源化研究等工作,E-mail:50250667@qq.com。
