摘要
为满足清醒小鼠的脑成像需求,研发了一种小鼠脑固定装置,并基于该装置建立了一套双光子显微镜活体小鼠脑样本制备方法。该方法涵盖小鼠麻醉和固定、颅骨开窗、头部固定器安装等关键步骤。利用此方法制备的小鼠脑样本,在双光子显微镜下观测脑内钙信号、血管网络及神经元结构时,可获得高信噪比的清晰图像。该活体小鼠脑样本制备方法为深入探究生理状态下鼠脑结构与功能提供了有力支撑。
To meet the imaging needs of awake mice,a novel mouse brain fixation device was independently developed.A detailed method for brain sample preparation and optimization was established based on this device,which is suitable for two-photon microscopic brain imaging experiments in awake mice.When we study signals such as intracranial calcium signals,vascular networks,and neuronal structures,we are possible to obtain clear images with a high signal-to-noise ratio.The method for preparing brain samples from living mice provides strong support for in-depth exploration of the structure and function of the mouse brain under physiological conditions.
作者
刘双双
刘方玥
毕超
洪晓黎
方三华
沈逸
LIU Shuangshuang;LIU Fangyue;BI Chao;HONG Xiaoli;FANG Sanhua;SHEN Yi(Core Facilities of School of Medicine,Zhejiang University,Hangzhou 310058,China;School of Brain Science and Brain Medicine,Zhejiang University,Hangzhou 310058,China)
出处
《实验室研究与探索》
北大核心
2025年第8期30-33,共4页
Research and Exploration In Laboratory
基金
浙江省教育厅一般科研项目(Y202250052)
浙江大学实验技术研究项目(SYBJS202202)。
作者简介
刘双双(1980-),女,山东滨州人,高级实验师,研究方向为共聚焦和双光子显微镜技术的开发。Tel.:0571-88206270,E-mail:liushuangshuang2008@126.com。
