摘要
为探究水分胁迫下外源CaCl_(2)对亚麻幼苗生长及生理的影响,设置0(CK)、1(Ca1)、3(Ca3)、6(Ca6)、12(Ca12)和24 mmol/L(Ca24)等6个CaCl2浓度处理,采用盆栽控水法,于枞形期进行根施处理。结果表明,与CK相比,Ca6与Ca12处理对胁迫下亚麻各形态参数增加的范围是27.10%~108.44%(干旱)和15.13%~66.29%(水淹);Ca6与Ca12处理在胁迫结束时均使亚麻的超氧化物歧化酶和过氧化物酶活性降低,而在干旱胁迫下增加了苯丙氨酸解氨酶(PAL)和过氧化氢酶(CAT)活性,在水淹胁迫下增加了CAT活性和降低了PAL活性;Ca6和Ca12处理显著降低了干旱胁迫与水淹胁迫下的超氧阴离子、丙二醛和过氧化氢含量,降低范围分别为24.14%~98.18%(干旱)和25.16%~76.17%(水淹)。变异系数分析表明,Ca6处理的综合影响最大,分别为20.07(干旱)和25.11(水淹)。隶属函数分析表明,Ca6处理的平均得分最高,分别为0.75(干旱)和0.63(水淹)。综上,6~12 mmol/L CaCl_(2)预处理亚麻幼苗可有效缓解水分胁迫对其形态和生理的不利影响,其中6 mmol/L的CaCl_(2)是最适预处理浓度。同时,本研究也证明了CaCl_(2)缓解干旱和水淹胁迫的最适浓度是同一浓度。
In order to investigate the effects of exogenous CaCl_(2) on the growth and physiology of flax seedlings under water stress,there are six different CaCl2 concentrations were set up,including 0(CK),1(Ca1),3(Ca3),6(Ca6),12(Ca12)and 24 mmol/L(Ca24).The pot water control method was adopted and the root application treatment was carried out at the fir stage.The results showed that,compared with CK,the ranges of increase in various morphological parameters of flax under stress by Ca6 and Ca12 treatments were 27.10%-108.44%(drought)and 15.13%-66.29%(waterlogging).Both Ca6 and Ca12 treatments reduced the superoxide dismutase and peroxidase activities of flax at the end of stress,while increased the phenylalnine ammonialyase and catalase(CAT)activities under drought stress and increased the CAT activity and reducing the PAL activity under waterlogging stress.Compared with CK,Ca6 and Ca12 treatments significantly reduced the contents of superoxide anion,malondialdehyde and hydrogen peroxide under drought stress and waterlogging stress,with the reduction ranges being 24.14%-98.18%(drought)and 25.16%-76.17%(waterlogging),respectively.The coefficient of variation analysis showed that the combined effects of Ca6 treatment were 20.07(drought)and 25.11(water flooding),respectively.Membership function analysis showed that the average scores of Ca6 treatment were the highest,0.75(drought)and 0.63(water flooding),respectively.In conclusion,6 and 12 mmol/L CaCl_(2) pretreatment of flax seedlings can effectively alleviate the adverse effects of water stress on the morphology and physiology of flax seedlings,and 6 mmol/L CaCl_(2) is the most suitable concentration for pretreatment.At the same time,this study also proved that the optimal concentration of CaCl_(2) to alleviate drought and flooding stress is the same concentration.
作者
褚霈宇
韩喜财
王盼
李多
朱浩
金喜军
宋鑫玲
曹洪勋
夏尊民
王晓楠
Chu Peiyu;Han Xicai;Wang Pan;Li Duo;Zhu Hao;Jin Xijun;Song Xinling;Cao Hongxun;Xia Zunmin;Wang Xiaonan(Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163319,Heilongjiang,China;Daqing Agricultural Technology Extension Center,Daqing 163411,Heilongjiang,China;College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163000,Heilongjiang,China)
出处
《作物杂志》
北大核心
2025年第4期206-213,共8页
Crops
基金
黑龙江省科学院青年创新基金项目(CXMS2024DQ04)
黑龙江省麻类作物(工业)现代产业技术协同创新技术体系[SCXTI(MLGY)2024]
黑龙江省科学院科学研究基金项目(KY2022DQ02)。
关键词
氯化钙
水分胁迫
亚麻幼苗
抗性生理
形态
CaCl_(2)
Water stress
Flax seedling
Resistance physiology
Morphology
作者简介
褚霈宇,主要从事麻类作物逆境育种研究,E-mail:chupeiyv@163.com;通信作者:夏尊民,主要从事亚麻育种研究,E-mail:xiazunmin68@126.com;共同通信作者:王晓楠,主要从事麻类作物遗传育种、栽培及分子生物学研究,E-mail:wxn_fern@163.com。
