摘要
【目的】旨在探究外源MeJA对玫瑰黑斑病抗性的调控作用。【方法】以‘紫枝’玫瑰(Rosa rugosa‘Zi zhi’)为试验材料,设置对照组、接MeJA、接菌和接菌+MeJA 4组处理,筛选最适MeJA浓度。测定0 d和4 d的叶片相关生理指标并进行转录组测序分析。【结果】喷施外源MeJA提高了玫瑰对黑斑病的抗性,0.10 mmol/L MeJA处理叶片的病情指数较对照显著降低15.33%,诱导抗性效果最佳。接菌和接菌+MeJA组叶片苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)活性均显著提高,可溶性蛋白(SP)和可溶性糖(SS)的含量提高,丙二醛(MDA)与脯氨酸的含量显著降低。转录组测序获得2384个差异基因,GO和KEGG富集分析显示,这些基因在乙烯、脱落酸和茉莉酸信号通路中显著富集,还参与植物激素信号转导、植物与病原菌互作和MAPK信号等。【结论】外源喷施0.10 mmol/L MeJA通过调节抗病相关酶活性和渗透调节物质含量,提高玫瑰植株的黑斑病抗性。RNA-seq技术挖掘到同时响应MeJA和病害胁迫的差异表达基因与代谢通路,推测RrMAPK3、RrWRKY53、RrPR1、RrRbohD基因可能是抗病相关。
[Objective]Black spot disease is one of the main leaf diseases of Rosa rugosa,caused by Marssinina rosae,which seriously affects the quality and yield of R.rugosa.In order to explore the regulation of exogenous MeJA on the resistance of R.rugosa black spot disease.[Methods]In this study,the effects of different concentrations of exogenous Methyl jasmonate(MeJA)on the resistance of R.rugosa to black spot disease were investigated,and the physiological changes and molecular pathways of R.rugosa in response to black spot disease infection were explored by means of physiology and transcriptomics,so as to provide a theoretical basis for the application of MeJA in the prevention and treatment of black spot disease.Rosa rugosa'Zi zhi'was used as the experimental material,and four treatments were set up,including control group,MeJA inoculation group,inoculation group and inoculation+MeJA group,to screen the optimal MeJA concentration.The physiological indexes of leaves at 0d and 4d were determined and transcriptome sequencing analysis was performed.[Results]Spraying exogenous MeJA increased the resistance of R.rugosa to black spot disease.The disease index of leaves treated with 0.10 mmol/L MeJA was significantly lower than that of the control by 15.33%,and the effect of inducing resistance was the best.The activities of phenylalanine ammonia lyase(PAL),peroxidase(POD),superoxide dismutase(SOD)and polyphenol oxidase(PPO)in the leaves of the inoculation and inoculation+MeJA groups were significantly increased,the contents of soluble protein(SP)and soluble sugar(SS)were increased,the content of malondialdehyde(MDA)and proline was significantly decreased.Transcriptome sequencing obtained 2384 differential genes.GO and KEGG enrichment analysis showed that the differentially expressed genes were significantly enriched in ethylene,abscisic acid and jasmonic acid signaling pathways,and were involved in phytohormone signaling transduction,host-pathogen interaction and mitogen-activated protein kinase(MAPK)signaling pathways.[Conclusion]Exogenous spraying of 0.10 mmol/L MeJA could improve the black spot resistance of R.rugosa plants by regulating the activity of disease resistance-related enzymes and the content of osmotic adjustment substances.After sequencing by RNA-seq technology,differentially expressed genes and metabolic pathways in response to MeJA and disease stress were discovered.It was speculated that RrMAPK3,RrWRKY53,RrPR1,and RrRbohD genes may be disease resistance-related genes.
作者
褚春雪
杨孜竞
仝伯强
鲁仪增
刘丹
臧德奎
吴其超
马燕
臧凤岐
CHU Chunxue;YANG Zijing;TONG Boqiang;LU Yizeng;LIU Dan;ZANG Dekui;WU Qichao;MA Yan;ZANG Fengqi(Forestry College of Shandong Agricultural University,Forestry Key Laboratory of the State Forestry and Grassland Administration of the Lower Yellow River,Tai'an,Shandong 271018,China;Shandong Forest and Grass Germplasm Resource Center,Jinan,Shandong 250102,China)
出处
《北京农学院学报》
2025年第3期1-14,共14页
Journal of Beijing University of Agriculture
基金
山东省重点研发计划(重大科技创新工程)项目“林木种质资源挖掘与精准鉴定”(2021LZGC023)
山东省重点研发计划项目(2024LZGC003)。
关键词
玫瑰
黑斑病
茉莉酸甲酯
转录组学
诱导抗病
Rosa rugosa
black spot disease
methyl jasmonate
inducing disease resistance
transcriptomics
作者简介
第一作者:褚春雪(2000-),女,山东济南人,硕士,研究方向为分子植物育种,E-mail:525192751@qq.com;通信作者:臧凤岐(1994-),女,山东泰安人,讲师,农学博士,主要从事林木种质资源与新品种专业研究,E-mail:1491887685@qq.com。
