摘要
目的探讨胶体金法检测甲型、乙型流感病毒的临床价值。方法选取2023年11月—2024年6月在四川省第三人民医院就诊的200例疑似流感患者,均采集口咽拭子标本,分别采用胶体金法和实时荧光定量多聚酶链式反应(qPCR)法进行检测,并评估两种检测方法结果的一致性。结果以qPCR法检测结果作为“金标准”,胶体金法检测甲型流感病毒的敏感度为58.62%、特异度为100%、阳性预测值为100%、阴性预测值为93.44%、准确率为94.00%,检测乙型流感病毒的敏感度为38.89%、特异度为100%、阳性预测值为100%、阴性预测值为94.30%、准确率为94.50%。Kappa检验结果显示,两种检测方法检测甲型、乙型流感病毒的一致性均一般(Kappa=0.708、0.537)。结论胶体金法相较于qPCR法,其检测甲型和乙型流感病毒的敏感度较低,特异度较高,两者的检测一致性均一般。在特定的医疗环境下,可首选胶体金法用于甲型、乙型流感的诊断,从而优化医疗资源的使用。
Objective To explore the clinical value of colloidal gold method in the detection of influenza A and B viruses.Methods A total of 200 patients with suspected influenza who were admitted to the Third People's Hospital of Sichuan Province from November 2023 to June 2024 were selected.Oropharyngeal swab specimens were collected and detected by colloidal gold method and quantitative real-time polymerase chain reaction(qPCR)method respectively,and the consistency of the results of the two detection methods was evaluated.Results The results of qPCR were used as the'gold standard.The sensitivity,specificity,positive predictive value,negative predictive value and accuracy of colloidal gold method for detecting influenza A virus were 58.62%,100%,100%,93.44%and 94.00%,respectively.The sensitivity,specificity,positive predictive value,negative predictive value and accuracy of colloidal gold method for detecting influenza B virus were 38.89%,100%,100%,94.30%and 94.50%,respectively.Kappa test results showed that the consistency of the two detection methods for influenza A and B viruses was general(Kappa=0.708,0.537).Conclusions Compared with the qPCR method,the colloidal gold method has lower sensitivity and higher specificity in detecting influenza A and B viruses,and the detection consistency of the two methods is general.In a specific medical environment,colloidal gold method can be preferred for the diagnosis of influenza A and B,so as to optimize the use of medical resources.
作者
孙锦秋
罗志刚
SUN Jinqiu;LUO Zhigang(Department of Laboratory Medicine,the Third People's Hospital of Sichuan Province,Chengdu,Sichuan 610000,China)
出处
《医药前沿》
2025年第23期65-68,共4页
Journal of Frontiers of Medicine
