摘要
为得到高转化率稀有人参皂苷益生菌,该研究将某菌种库自行分离所得益生菌冻干粉经过活化、分离、培养后利用七叶苷显色法初筛得到18株产β-葡萄糖苷酶益生菌菌株,使用熊果苷进行碳源诱导筛选出3株高产β-葡萄糖苷酶益生菌菌株,并利用此3株菌株对人参进行发酵,得到1株高转化率稀有人参皂苷菌株FTCM001。该菌株发酵使得稀有人参皂苷Rg3含量提高0.020 mg/mL,较发酵前提升66.7倍。经过形态观察、16S rRNA基因测序、pheS基因测序和生理生化特征分析鉴定,该菌株被鉴定为植物乳植杆菌(Lactiplantibacillus plantarum)。
In order to obtain rare ginsenoside probiotics with a high conversion rate,this study activated,separated,and cultivated the probiotic freezedried powder obtained from a selfseparation bacterial strain library and then used the esculin plate colored method for preliminary screening,finally obtaining 18 probiotic strains that producedβ-glucosidase.Arbutin was used for carbon source induction screening,and three strains with highβ-glucosidase production were selected as probiotic strains.These three strains were then used for the fermentation of ginseng,resulting in a rare ginsenoside strain FTCM001 with a high conversion rate.After fermentation of this strain,the content of rare ginsenoside Rg3 increased by 0.020 mg/mL,representing a 66.7fold increase compared to the pre-fermentation level.After morphological observation,16S rRNA gene sequence,pheS gene sequence,and physiological and biochemical characteristic analysis and identification,this strain was identified as Lactiplantibacillus plantarum.
作者
崔硕
沈晓勇
林峰
常世敏
CUI Shuo;SHEN Xiaoyong;LIN Feng;CHANG Shimin(School of Life Science and Food Engineering,Hebei University of Engineering,Handan 056038,Hebei,China;Handan Key Laboratory of Natural Products and Functional Foods,Handan 056038,Hebei,China;Jiangsu Junyao Life Science and Technology Development Co.,Ltd.,Yancheng 224100,Jiangsu,China)
出处
《食品研究与开发》
2025年第15期159-165,共7页
Food Research and Development
基金
河北省体育局体育科技研究项目(2024JT04)。
关键词
稀有人参皂苷
筛选
鉴定
益生菌菌株
发酵
rare ginsenoside
screening
identification
probiotic strain
fermentation
作者简介
崔硕(2001-),男(汉),硕士研究生,研究方向:功能性食品研发;通信作者:林峰,高级工程师;常世敏(1977-),女,教授,博士,研究方向:功能性食品研发。
