摘要
A型多杀性巴氏杆菌(Pasteurella multocida,Pm)是引起牛肺炎等呼吸系统疾病的重要病原菌,严重制约我国养牛业的健康发展。目前,主要通过疫苗接种预防该病,但由于Pm血清型众多,导致疫苗交叉免疫保护作用较差。因此,研发具有交叉保护作用的疫苗对防控Pm感染具有重要意义。本课题组前期研究发现,PmCQ2Δcra对A、B、F等不同荚膜血清型菌株(PmA、PmB、PmF)均具有良好的免疫保护作用。转录组学测序分析结果提示,PmCQ2Δcra良好的交叉免疫保护作用可能与细菌表面保护性抗原的高表达有关。因此,本研究通过生物信息分析筛选并表达了4个假定免疫保护性抗原蛋白PmCQ2-5175、PmCQ2-6290、PmCQ2-0275和PmCQ2-2640,结果显示,这些假定抗原制备的亚单位疫苗对PmA感染小鼠的保护效力分别为62.5%、25%、12.5%和10%。其中,保护性最佳的单组分抗原疫苗PmCQ2-5175对PmB感染小鼠具有75%的交叉保护作用。此外,本研究筛选的PmCQ2-5175蛋白保护性优于已报道的Pm抗原蛋白plpE,且融合表达蛋白PmCQ2-5175-plpE对PmA的保护性优于单一蛋白。本研究为开发具有交叉免疫保护作用的Pm亚单位疫苗提供了重要的抗原靶标。
Pasteurella multocida(Pm)type A is an important pathogen responsible for respiratory diseases,such as bovine pneumonia,which seriously restricts the development of cattle industry in China.Currently,the prevention of Pm infection-related diseases primarily relies on vaccination in production.However,the diverse Pm serotypes result in inadequate cross-immunological protection from vaccines.Therefore,it is of great significance to develop vaccines with cross-protection for the prevention and control of Pm infectious diseases.The previous studies conducted by our team have demonstrated that PmCQ2△cra exhibits a strong immune protective effect against Pm type A(PmA),Pm type B(PmB),and Pm type F(PmF).Transcriptomic sequencing results suggest that the cross-immunoprotective effect of PmCQ2△cra may be attributed to high expression levels of bacterial surface protective antigens.Consequently,four putative immune protective antigens,namely PmCQ2-5175,PmCQ2-6290,PmCQ2-0275 and PmCQ2-2640,were screened through bioin-formatics analysis in this study.Subunit vaccines formulated with these potential antigenic proteins exhibited protective efficacy of 62.5%,25%,12.5%and 10%against PmA-infected mice,respectively.Importantly,PmCQ2-5175,one of the most protective single-component antigen vac-cines,demonstrating a 75%cross-protection against PmB infection in mice.Furthermore,the pro-tective efficacy of the PmCQ2-5175 protein screened in this study was superior to that of the previ-ously reported Pm antigen protein plpE.Moreover,the fusion expression protein PmCQ2-5175-PLPE exhibited better protective effects against PmA compared to a single protein.The findings of this study will establish a theoretical foundation for the advancement of Pm subunit vaccines with broad-spectrum immune protection.
作者
熊盼
黄艳澜
刘思渝
杨柳
赵光夫
李能章
何芳
彭远义
XIONG Pan;HUANG Yanlan;LIU Siyu;YANG Liu;ZHAO Guangfu;LI Nengzhang;HE Fang;PENG Yuanyi(College of Veterinary Medicine,Southwest University,Beibei,Chongqing 400715,China)
出处
《中国兽医学报》
北大核心
2025年第5期963-970,共8页
Chinese Journal of Veterinary Science
基金
中央高校基本科研业务费专项资金资助项目(SWU-KQ22068)
重庆市博士后特别资助项目(2023WBSHTB2030)
财政部和农业农村部国家现代农业(肉牛牦牛)产业技术体系资助项目(CARS-37)。
关键词
多杀性巴氏杆菌
免疫保护
重组蛋白
小鼠
Pasteurella multocida
immune protection
recombinant protein
mice
作者简介
熊盼(1998-),女,硕士研究生;通信作者:何芳,E-mail:hefang2017@sina.com;通信作者:彭远义,E-mail:pyy2002@sina.com。
