摘要
目的乌天麻为高海拔产名贵中药材,目前缺乏温度和种子成熟度对其种子萌发影响的研究。阐明这两种因素对种子萌发的影响,对乌天麻有性繁殖防止天麻退化有重要理论和实际应用价值。方法本实验对以乌天麻为主的3种天麻变型进行2,3,5-三苯基氯化四氮唑(TTC)染色和低温共生萌发实验,测定不同成熟度种子的性状和萌发指标,采用主成分分析和隶属函数值法对种子活力指标进行综合评价分析,并采用冗余分析(RDA)揭示种子萌发和温度及种子成熟度的相关性。结果乌天麻和绿天麻种子细长型,红天麻短粗型,且乌天麻种胚体积最大;不同天麻的低温共生萌发结果存在差异(P<0.05)。结论温度显著影响种子萌发,而成熟度影响相对较小,结合综合评价值,明确了乌天麻最优萌发组合为16℃-将裂果,为乌天麻规模化生产提供了科学依据。
OBJECTIVE To elucidate the effects of temperature and seed maturity on seed germination of Gastrodia elata Bl.f.glauca and provide theoretical and practical value for the sexual reproduction of G.elata Bl.f.glauca and the prevention of its degen-eration.METHODS 2,3,5-triphenyltetrazolium chloride(TTC)staining and low-temperature symbiotic germination experiments were performed on three formae of G.elata(G.elata Bl.f.glauca as the primary focus).Seed traits and germination indices were quantified across maturity stages.A combined approach of Principal Component Analysis(PCA)and membership function valuation was applied for comprehensive seed vigor assessment.Furthermore,Redundancy Analysis(RDA)was utilized to reveal the correla-tions among seed germination,temperature,and seed maturity.RESULTS The seeds of G.elata Bl.f.glauca and G.elata Bl.f.viridis exhibited an elongated morphology in contrast to the shorter and thicker seeds of G.elata Bl.f.elata,and G.elata Bl.f.glau-ca possessed the largest embryo volume among the variants.The low-temperature symbiotic germination outcomes varied significantly a-mong the different G.elata variants(P<0.05).CONCLUSION Temperature significantly affects seed germination,whereas the impact of maturity is relatively minor.Based on the comprehensive evaluation values,the optimal germination combination for G.elata Bl.f.glauca is determined to be 16℃-near-dehiscent fruit,providing a scientific basis for the large-scale production of G.elata Bl.f.glauca.
作者
刁海欣
郭顺星
DIAO Haixin;GUO Shunxing(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)
出处
《中国药学杂志》
北大核心
2025年第12期1247-1254,共8页
Chinese Pharmaceutical Journal
基金
国家自然科学基金项目资助(82373988)
中国医学科学院医学与健康科技创新工程项目资助(2021-I2M-1-031)。
关键词
乌天麻
温度
成熟度
共生萌发
综合评价
Gastrodia elata Bl.f.glauca
temperature
maturity
symbiotic germination
comprehensive evaluation
作者简介
刁海欣,女,博士研究生研究方向:药用植物菌根生物学;通信作者:郭顺星,男,博士,教授研究方向:药用植物菌根生物学,Tel:(010)57833231。
