摘要
目的探讨不同波长发光二极管(LED)对大肠埃希菌(E.coli)的杀菌效果及作用机制。方法以E.coli为研究对象,通过LED波长将其分为对照组、405 nm组、415 nm组和430 nm组,LED功率均为50 mW·cm^(-2)。通过OD_(600)值、菌落计数评估杀菌效果;扫描电镜观察细菌形态变化,荧光探针检测活性氧水平,紫外分光光度法分析细胞膜通透性,琼脂糖凝胶电泳评估DNA损伤。结果415 nm LED照射30 min杀菌效果最佳,菌落数较对照组下降2.3log_(10)(CFU·mL^(-1)),405 nm组菌落数下降1.4log_(10)(CFU·mL^(-1))(P均<0.01)。415 nm LED可诱导E.coli产生大量活性氧,OD_(260)、OD_(280)处吸光度值显著升高,并引起DNA降解;E.coli细胞菌体皱缩、破裂。结论415 nm LED通过激发内源性光敏剂,诱导活性氧生成、破坏细胞膜及DNA发挥杀菌作用。
Objective To explore the bactericidal effect and mechanism of light-emitting diode(LED)with different wavelengths on Escherichia coli.Methods E.coli was used as the target of the study.The study was divided into a control group and groups with different wavelengths of LED of 405,415,and 430 nm.The LED power was 50 mW·cm^(-2).The bactericidal effect was evaluated by OD_(600) and colony counting.Changes in bacterial morphology were observed using scanning electron microscopy,reactive oxygen species(ROS)levels were detected using a fluorescent probe,cell membrane permeability was analyzed using UV spectrophotometry,and DNA damage was assessed using agarose gel electrophoresis.Results The best bactericidal effect was achieved by irradiating with 415 nm LED for 30 minutes.The colony count decreased by 2.3 log_(10)(CFU·mL^(-1))compared with the control group.The colony count in the 405 nm group decreased by 1.4 log_(10)(CFU·mL^(-1)),the differences were all statistically significant(P all<0.01).Mechanism studies showed that 415 nm LED induced E.coli to produce a large amount of ROS,significantly increasing the absorbance values at OD_(260) and OD_(280) and causing DNA degradation.And the E.coli cell bacterial body was wrinkled and ruptured.Conclusion 415 nm LED played a bactericidal role by stimulating the endogenous photosensitizer,resulting in the generation of ROS,damaging the cell membrane and DNA.
作者
陈修鑫
杨延辉
马远
张瑞琪
祁馨仪
李博巍
哈春芳
CHEN Xiuxin;YANG Yanhui;MA Yuan;ZHANG Ruiqi;QI Xinyi;LI Bowei;HA Chunfang(The First Clinical Medical College of Ningxia Medical University,Yinchuan 750004,China;Ningxia Clinical and Pathogenic Microbiology Laboratory,Yinchuan 750004,China;General Hospital of Ningxia Medical University,the First Clinical Medical College of Ningxia Medical University,Yinchuan 750004,China;Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education,Ningxia Medical University,Yinchuan 750004,China)
出处
《宁夏医科大学学报》
2025年第6期562-566,共5页
Journal of Ningxia Medical University
基金
国家重点研发计划项目(2022YFB3604704)
宁夏回族自治区教育厅高等学校科学研究项目(NYG2024122)。
关键词
发光二极管
大肠埃希菌
细菌性阴道病
杀菌机制
light-emitting diode
Escherichia coli
bacterial vaginosis
bactericidal mechanism
作者简介
陈修鑫(1999-),在读硕士研究生,研究方向为妇产科学。E-mail:chen170104@163.com;通信作者:哈春芳,女,博士研究生导师,主任医师,研究方向为妇科肿瘤、子宫内膜异位症。E-mail:hachunfang@163.com。
