摘要
为探索一种较理想的辐射防护替代方案,本研究对刺梨浓缩汁中的主要成分——VC和总黄酮进行了定量(VC质量分数≥2.5%,总黄酮质量分数≥2%),并以刺梨浓缩汁为实验对象,采用1,1-二苯基-2-三硝基苯肼自由基、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基和羟自由基清除实验测定其抗氧化能力,通过体外细胞实验和动物实验评估其辐射防护效果。结果显示,刺梨浓缩汁对3种自由基均具有一定的清除能力,且能显著改善由电离辐射导致的HL-7702细胞活性下降。与辐射模型组小鼠相比,持续服用30 d刺梨浓缩汁(剂量1.0 g/kg)小鼠的脾脏指数、血清和脾脏中超氧化物歧化酶活性、血清和肝脏中丙二醛含量以及血清、肝脏、脾脏中谷胱甘肽含量均得到显著改善,同时对辐射导致的肝功能损伤也有一定的保护作用。本研究明确了刺梨浓缩汁发挥辐射防护作用的主要功效成分及起效量,可为日后刺梨浓缩汁品质分级标准的制定以及功能食品的开发提供依据。
To explore an ideal alternative approach for radiation protection,this study quantified vitamin C(≥2.5%)and total flavonoids(≥2%)in Rosa roxbunghii Tratt.juice concentrate.Its antioxidant capacity was evaluated using 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical cation,and hydroxyl radical scavenging assays,and its efficacy in radiation protection was assessed by cell and animal experiments.Results showed that R.roxbunghii Tratt.juice concentrate scavenged all three free radicals and significantly suppressed the reduction in HL-7702 cell viability caused by ionizing radiation.Compared with the radiation model group,the spleen index and superoxide dismutase(SOD)activity in the serum and spleen,the malondialdehyde(MDA)content in the serum and liver,and the glutathione content in the serum,liver and spleen of mice treated with 1.0 g/kg of R.roxbunghii Tratt.juice concentrate for 30 days were significantly alleviated,and it also had a protective effect against liver function damage caused by radiation.This study lay the basis for establishing quality grading standards for R.roxbunghii Tratt.juice concentrate and for its application to functional foods.
作者
邝铭晴
贝琦
刘迎欣
朱家庆
李雪峰
伍柏坚
鲁吉珂
郝利民
KUANG Mingqing;BEI Qi;LIU Yingxin;ZHU Jiaqing;LI Xuefeng;WU Bojian;LU Jike;HAO Limin(China Key Laboratory of Light Industry Plant Beverage,Guangzhou 510623,China;School of Life Science,Zhengzhou University,Zhengzhou 450001,China;School of Food Science and Engineering,South China University of Technology,Guangzhou 510641,China;Quartermaster Engineering Technology Research Institute,Systems Engineering Research Institute,Academy of Military Sciences,Beijing 100010,China)
出处
《食品科学》
北大核心
2025年第16期183-193,共11页
Food Science
基金
国家自然科学基金面上项目(32172211)
中原科技创新领军人才项目(254000510040)。
关键词
刺梨浓缩汁
VC
黄酮
抗氧化
电离辐射
Rosa roxbunghii Tratt.juice concentrate
vitamin C
flavonoids
antioxidant
ionizing radiation
作者简介
第一作者:邝铭晴(1997-)(ORCID:0009-0008-8200-4182),女,工程师,硕士,研究方向为天然产物的开发和利用,E-mail:364347128@qq.com;通信作者:鲁吉珂(1982-)(ORCID:0000-0001-9339-4835),男,教授,博士,研究方向为食品生物技术,E-mail:ljk002004@163.com;通信作者:郝利民(1969-)(ORCID:0000-0003-3098-5149),男,教授,博士,研究方向为军用功能食品与食品生物技术,E-mail:hlm2005@163.com。
