摘要
为了提高纤维素发酵产氢效率,该研究以微晶纤维素为限制性底物,牛粪堆肥为菌源,经过富集驯化培养获得可以高效降解纤维素产氢的嗜温产氢菌群,采用高通量测序技术对驯化菌群的细菌菌群结构进行分析,并在单因素试验的基础上,通过响应面试验优化发酵工艺。结果表明,经过30轮富集驯化培养获得了可以高效降解纤维素产氢的嗜温产氢菌群。驯化前后菌群结构和数量发生明显变化,主要细菌门为厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes),主要细菌属为梭菌属(Clostridium)、厌氧细杆菌属(Anaerofilum)和假单胞菌属(Pseudomonas),发酵体系内形成了纤维素降解菌、发酵产氢菌和纤维素降解产氢菌之间的生长协同作用,有效提高了纤维素降解和氢气的产生。驯化菌群发酵微晶纤维素产氢的最佳发酵工艺为碳酸氢铵质量浓度2.5 g/L、磷酸二氢钾质量浓度1.8 g/L、营养液添加量8.2 mL/L,在此优化条件下最大氢气产量可达(267.50±0.50)mL/g。
In order to improve the efficiency of hydrogen production from cellulose by fermentation,using microcrystalline cellulose as the limiting substrate,and cow manure compost as the bacterial source,the thermophilic hydrogen-producing flora that could efficiently degrade cellulose were obtained after enrichment and domestication,and the bacterial flora structure of the domesticated flora was analyzed by high-throughput sequencing technology.On the basis of single factor tests,the fermentation process was optimized by response surface tests.The results showed that the thermophilic hydrogen-producing bacteria,which could efficiently degrade cellulose to produce hydrogen,were obtained after 30 rounds of enrichment and domestication.The structure and quantity of the flora changed significantly before and after domestication.The main bacterial phyla were Firmicutes,Proteobacteria and Bacteroidetes,and the main bacterial genera were Clostridium,Anaerofilum and Pseudomonas.The growth synergy among cellulose-degrading bacteria,hydrogen-producing bacteria by fermentation and degrading cellulose was formed in the fermentation system,which effectively improved cellulose degradation and hydrogen production.The optimal fermentation process for hydrogen production from microcrystalline cellulose fermented by domesticated flora was ammonium bicarbonate mass concentration 2.5 g/L,potassium dihydrogen phosphate mass concentration 1.8 g/L,and nutrient solution addition 8.2 ml/L.Under these optimal conditions,the maximum hydrogen production reached(267.50±0.50)ml/g.
作者
刘前进
李甜甜
万帅
陈彪
张洁
蔡莉莉
李耀光
张元浩
巩笑楠
张靖楠
臧翔宇
LIU Qianjin;LI Tiantian;WAN Shuai;CHEN Biao;ZHANG Jie;CAI Lili;LI Yaoguang;ZHANG Yuanhao;GONG Xiaonan;ZHANG Jingnan;ZANG Xiangyu(China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450001,China;School of Food and Biological Engineering,ZhengzhouUniversity of Light Industry,Zhengzhou 450001,China;Henan Jinrui Flavoring and Essence Company,Zhengzhou 450000,China)
出处
《中国酿造》
北大核心
2025年第7期84-90,共7页
China Brewing
基金
国家自然科学基金项目(21706244)
河南中烟工业有限责任公司科技项目(AW2024008)。
关键词
微晶纤维素
牛粪堆肥
菌群驯化
氢气
发酵工艺
响应面法
microcrystalline cellulose
cow manure compost
microflora domestication
hydrogen
fermentation process
response surface method
作者简介
刘前进(1987-),男,工程师,硕士,研究方向为烟用香精香料的开发、应用及品质控制;通讯作者:臧翔宇(1994-),男,助理研究员,博士,研究方向为烟用香精香料的开发、应用及品质控制。
