摘要
抗菌肽是一类由原核生物和真核生物产生的小分子多肽,具有广谱抑菌活性,且不易引发微生物耐药性,因此被认为具有替代抗生素成为新一代抗菌药物的潜力。该文主要综述了抗菌肽的研究进展,并展望了其在医药、畜牧业、农业及食品领域中的应用前景。此外,该文还总结了近年来抗菌肽在毕赤酵母中的重组表达研究,并探讨了提高抗菌肽在该系统中高效表达的优化策略,包括启动子、信号肽、蛋白伴侣的选择、基因剂量及发酵参数的优化。希望通过这些总结,为抗菌肽的应用开发和大规模生产提供有价值的参考。
Antimicrobial peptides(AMPs)are small peptides produced by both prokaryotic and eukaryotic organisms,exhibiting broad-spectrum antibacterial activity and low potential for inducing microbial resistance.As such,AMPs are considered promising candidates to replace antibiotics as the next generation of antimicrobial agents.This paper reviews the recent progress in AMP research and explores their potential applications in medicine,animal husbandry,agriculture,and the food industry.Additionally,it summarizes recent studies on the recombinant expression of AMPs in Pichia pastoris and discusses strategies to enhance their expression efficiency in this system,including the selection of promoters,signal peptides,and molecular chaperones,gene dosage optimization and fermentation parameters.Through these insights,this review aims to provide valuable references for the development and large-scale production of AMPs.
作者
查曼
闵勇
刘晓艳
朱镭
邱一敏
陈凌
周荣华
王常高
ZHA Man;MIN Yong;LIU Xiaoyan;ZHU Lei;QIU Yimin;CHEN Ling;ZHOU Ronghua;WANG Changgao(School of Life and Health Science,Hubei University of Technology,Wuhan 430068,China;Hubei Biopesticide Engineering Research,Wuhan 430064,China;Institute of Plant Protection and Soil Fertilizer,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)
出处
《食品与发酵工业》
北大核心
2025年第13期354-361,共8页
Food and Fermentation Industries
基金
湖北省技术创新计划项目(2024BBB064)
湖北省现代农业产业技术体系项目(2023HBSTX4-07)
国家自然科学基金项目(32000052)。
关键词
抗菌肽
抗生素
微生物耐药性
毕赤酵母
重组表达
antimicrobial peptides
antibiotic
microbial resistance
Pichia pastoris
recombinant expression
作者简介
第一作者:查曼,硕士研究生;共同通信作者:周荣华研究员,E-mail:zhouronghua1975@126.com;共同通信作者:王常高副教授,E-mail:971963552@qq.com。
