摘要
本研究以铜绿微囊藻(Microcystis aeruginosa)为受试生物,探究薄层二硫化钼(MoS_(2))的水生毒性效应及相关作用机制.结果表明,不同浓度MoS_(2)处理组(0.01~50mg/L)在短期内(0~7d)抑制藻胞生长,其中在培养6d后50mg/L MoS_(2)对藻胞生长达到最高抑制作用(75%).在7d内MoS_(2)对藻胞色素、有机物合成表现出较强抑制作用(色素低于0.5mg/L,多糖和蛋白质低于0.05mg/mL,脂质低于2×10^(5)FU),且浓度越高抑制作用越强.随暴露时间延长至14~21d,抑制作用相对减轻,但高浓度处理组(50,10mg/L)仍对藻胞生长存在较强毒害作用.MoS_(2)光照产生ROS攻击藻胞使其发生急性/慢性氧化应激,0~7d内藻胞活性氧(ROS)、超氧化物歧化酶活性(SOD)以及胞外分泌物(EPS)升高,暴露14~21d后氧化应激水平逐渐恢复.然而,高浓度(50,10mg/L)暴露下,氧化应激反应超过藻胞的承载能力使其发生膜损伤.研究结果为MoS_(2)纳米材料的水生生态毒性和评估提供基础数据和理论支撑.
This study investigated the aquatic toxicity effects and underlying mechanisms of thin-layer molybdenum disulfide(MoS_(2))using the Microcystis aeruginosa as a model organism.The results demonstrated that various concentration of MoS_(2)(0.01~50mg/L)significantly inhibited algal growth during short-term exposure(0~7 days),with the highest inhibition rate of 75%observed in the 50mg/L group after 6days of cultivation.Throughout the 7-day exposure phase,MoS_(2) exhibited strong dose-dependent inhibitory effects on pigment biosynthesis and biomass accumulation,as evidenced by concentration-responsive reduction in pigment content(<0.5mg/L),polysaccharides and proteins(<0.05mg/mL),and lipids synthesis(<2×10^(5) fluorescence units).When exposure duration extended to 14~21 days,the observed inhibitory effects gradually alleviated,though high-concentration treatments(50 and 10mg/L)still maintained substantial growth inhibition.Mechanistic analysis revealed that photoactivated MoS_(2) nanoparticles generated reactive oxygen species(ROS),triggering acute/chronic oxidative stress in algal cells.During the initial 0~7 day exposure,intracellular ROS levels,superoxide dismutase(SOD)activity,and extracellular polymeric substances(EPS)secretion all significantly increased.These oxidative stress indicators progressively normalized during prolonged exposure(14~21 days).However,under high-concentration exposure conditions(50 and 10mg/L),the oxidative stress response exceeded cellular defense capacity,ultimately causing irreversible membrane damage.These findings provide fundamental data and theoretical support for ecological risk assessment of MoS_(2) nanomaterials in aquatic environments.
作者
马乙洮
盛科梦
胥志祥
李思沅
卢浩
李彩情
熊金瑞
潘学军
MA Yi-tao;SHENG Ke-meng;XU Zhi-xiang;LI Si-yuan;LU Hao;LI Cai-qing;XIONG Jin-rui;PAN Xue-jun(Faculty of Environmental Science and Engineering,Kunming University of Science and Technology,Kunming 650500,China;Southwest united Graduate School,Kunming 650092,China)
出处
《中国环境科学》
北大核心
2025年第7期3994-4002,共9页
China Environmental Science
基金
国家自然科学基金资助项目(42307500)
昆明理工大学分析测试基金资助项目(2023T20220174)
云南省大学生创新创业项目(S202210674068)。
关键词
铜绿微囊藻
纳米二硫化钼
氧化应激
生长抑制
生态风险
Microcystis aeruginosa
molybdenum disulfide
oxidative stress
growth inhibition
ecological risk
作者简介
马乙洮(2001-),女,云南大理人,昆明理工大学硕士研究生,主要从事纳米材料生态安全与健康风险研究.发表论文2篇.2942169341@qq.com;胥志祥,责任作者,副教授,xuzhixiang@kust.edu.cn。
