摘要
为了对疑似感染犬细小病毒(Canine parvovirus,CPV)的患犬进行确诊并了解感染毒株的遗传变异进化情况,通过临床症状和组织病理变化观察,结合病原核酸检测等方法鉴定病犬的致病原,并对感染CPV毒株的VP2基因进行扩增与测序、Blast比对分析、氨基酸位点分析和遗传演化分析。结果显示,肠内容物样品CPV胶体金试纸检测和CPV特异性PCR均为阳性,确定患犬死于CPV感染,命名该毒株为CPV-HN。随后对该毒株的VP2基因进行扩增,核苷酸序列分析结果显示CPV-HN株与CPV-HN1617株VP2基因相似性达100%,与其他参考毒株的同源性为97.7%到99.9%之间。氨基酸特异性位点分析表明,该毒株的426位点为谷氨酸;遗传演化分析表明,CPV-HN株与CPV-2c基因型毒株属于同一分支,为了解该地区CPV的流行情况提供了参考依据。
In order to investigate the causative agent of a suspected CPV infection case and elucidate the genetic evolution of circulating strain,this study employed a comprehensive approach integrating clinical symptom evaluation,histopathological examination,and molecular diagnostics,and the VP2 gene of the identified CPV strain was then amplified for subsequent sequencing,followed by BLAST analysis,amino acid mutation analysis and phylogenetic evolution analysis.The results of the colloidal gold immunochromatographic assay and CPV-specific PCR targeting intestinal contents confirmed were all positive,which providing conclusive evidence of lethal CPV infection,and named the infected strain as CPV-HN.Then,the VP2 gene of CPV-HN was amplified with a 1755 bp product and sequenced.The nucleotide sequence analysis demonstrated the VP2 gene of CPV-HN shared 100%identity with the CPV-HN1617 strain,while sharing 97.7-99.9%identity with other reference strains.Moreover,the amino acid mutation analysis revealed the VP2 protein of CPV-HN contains a characteristic glutamic acid at position 426,and the phylogenetic analysis showed that the CPV-HN was clustered within the CPV-2c genotype clade,which provides reference for understanding the prevalence of CPV in this region.
作者
贾文亮
杨文迪
李懿
张馨月
黄文卓
曹晶
朱彦龙
南卫星
李新锋
张晓战
JIA Wenliang;YANG Wendi;LI Yi;ZHANG Xinyue;HUANG Wenzhuo;CAO Jing;ZHU Yanlong;NAN Weixing;LI Xinfeng;ZHANG Xiaozhan(Agriculture and Rural Bureau of Huixian,Xinxiang Henan 453600;College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou Henan 450046;Shaoxing Jiawen Pet Hospital Co.,Ltd,Shaoxing Zhejiang 312000;Boda Pet Hospital,Sanmenxia Henan 472100;Livestock Product Quality and Safety Technology Research Institute,Henan University of Animal Husbandry and Economy,Zhengzhou Henan 450046)
出处
《现代牧业》
2025年第2期6-11,共6页
Modern Animal Husbandry
基金
河南省自然科学基金面上项目(232300420028)
河南省科技攻关项目(242102110058)
河南省重点学科“兽医”项目(312)。
作者简介
贾文亮(1991-),男,河南新乡人,助理兽医师,硕士生。;通信作者:张晓战(1987-),男,河南禹州人,副教授,博士。
