摘要
对杏鲍菇菌株进行种质资源多样性研究,筛选出具有生育期短、产量高的优良菌株作为工厂化育种的试验材料,为杏鲍菇育种手段和方法的深入研究及开发利用提供参考。以国内收集的34株杏鲍菇菌株(B1-B34)为试验材料,对体细胞不亲和性试验、ISSR分子标记技术、农艺性状评价进行比较分析及优良杂交菌株选育。结果表明,参与试验的34株杏鲍菇菌株遗传变异丰富,遗传相似系数的变化范围为0.73~0.97,当遗传相似性系数为0.85时,可将34株杏鲍菇菌株分为4类。农艺性状分析结果表明,产量最高的杏鲍菇菌株是B2,产量最低的是杏鲍菇B7。生育期最短的杏鲍菇菌株是B1,只需要63 d,生育期最长的杏鲍菇菌株是B11、B14和B30,均为80 d。综合以上结果,筛选出产量高、生物学效率高的B2菌株与生长周期短的B1菌株作为杂交育种亲本,最终筛选出优良杂交菌株LX119,该菌株生育期为56 d、产量为762.14 g·袋^(-1)。
To analyze the diversity of germplasm resources of Pleurotus eryngii strains,this study screened excellent strains with short growth period and high yield as industrial breeding materials,aiming to provide theoretical basis for the in-depth development of P.eryngii breeding technology.In this study,34 strains of P.eryngii(B1-B34)collected domestically were used as test materials,and the somatic cell incompatibility experiment,ISSR molecular marker technology,and agronomic trait evaluation were compared and analyzed,and the excellent hybrid strains were selected.The results showed that the 34 strains of P.eryngii were rich in genetic variation,with a genetic similarity coefficient from 0.73-0.97.When the genetic similarity coefficient was 0.85,the 34 strains of P.eryngii could be classified into four categories.The agronomic traits showed that the highest total yield was B2,and the lowest yield was B7.The shortest growth period was B1,which took only 63 days,while the longest growth period was B11,B14 and B30,all of which took 80 days.Based on the analysis of the above results,the B2 strain with high yield and high biological efficiency and the B1 strain with short growth cycle were selected as hybrid breeding parents.Finally,the excellent hybrid strain LX119 was screened,with a growth period of 56 days and a yield of 762.14 g·bag^(-1).
作者
刘汶鑫
王恬恬
唐玉琴
梁运江
LIUWenxin;WANG Tiantian;TANG Yuqin;LIANG Yunjiang(Yanbian University,Yanji 133000,Jilin,China;Jilin Agricultural Science and Technology College,Jilin 132101,Jilin,China)
出处
《中国瓜菜》
北大核心
2025年第7期109-126,共18页
China Cucurbits And Vegetables
基金
吉林省科技发展计划项目(20220202102NC)。
关键词
杏鲍菇
ISSR分析
拮抗反应
农艺性状
杂交育种
Pleurotus eryngii
ISSR analysis
Antagonistic reaction
Agronomic trait
Cross breeding
作者简介
刘汶鑫,女,在读硕士研究生,研究方向为农业环境与生物质综合利用。E-mail:1981317340@qq.com;通信作者:唐玉琴,女,教授,主要从事真菌资源开发与利用方面教学和研究。E-mail:335673084@qq.com;通信作者:梁运江,男,教授,主要从事土壤学等研究工作。E-mail:lyjluo@ybu.edu.cn。
