摘要
目的比较18种菌株和2种指示微生物对含氯、过氧化氢及季铵盐类消毒剂的抗性差异。方法选取食品、环境和人体来源的革兰氏阴性菌、革兰氏阳性菌及芽孢菌3类菌株(每类6种),经基质辅助激光解吸电离飞行时间质谱仪(matrix-assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF MS)和生化鉴定种属。以金黄色葡萄球菌ATCC6538和大肠杆菌80992种指示微生物为参考菌株,制备2.0×10^(8)~10.0×10^(8) CFU/mL菌悬液,检测其对次氯酸钠(有效氯0.00400%~0.00499%)、过氧化氢(7.5%)及苯扎溴铵(3.996 mg/mL)的耐受性,作用时间均为3 min。通过计算消毒剂对不同菌株的杀灭对数值(killing log value,KL),比较不同种属菌株对3种消毒剂抗性差异。结果消毒剂杀菌效力从强到弱为:次氯酸钠、过氧化氢、苯扎溴铵;不同菌种抗性存在显著差异,且常见的3类菌株(革兰氏阴性菌、革兰氏阳性菌及芽孢菌)中,均有对消毒剂抗性强于2种指示微生物的菌株。结论次氯酸钠与过氧化氢的杀菌效果优于苯扎溴铵;菌株抗性具有种属特异性,不能单纯通过革兰氏分类预判;指示微生物应依据实际需求扩大筛选范围。
Objective To compare the resistance differences of 18 kinds of bacterial strains and 2 kinds of biological indicators to chlorine-containing disinfectants,hydrogen peroxide and quaternary ammonium compounds.Methods Strains were selected from food,environment and human sources,including gram-negative bacteria,gram-positive bacteria and spore-forming bacteria(6 kinds of strains per category).Species identification was performed using matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS)and biochemical assays.The 2 kinds of biological indicators,namely Staphylococcus aureus ATCC6538 and Escherichia coli 8099 were used as reference strains.Bacterial suspensions(2.0×10^(8)-10.0×10^(8) CFU/mL)were prepared to test their tolerance to sodium hypochlorite(available chlorine 0.00400%-0.00499%),hydrogen peroxide(7.5%),and benzalkonium bromide(3.996 mg/mL).All bacterial strains were treated with disinfectants for 3 minutes.By calculating the killing log value(KL)of disinfectants against different bacterial strains,the differences in resistance of different species of bacterial strains to 3 kinds of disinfectants were compared.Results The bactericidal efficacy ranked as sodium hypochlorite,hydrogen peroxide,benzalkonium bromide.Different species showed significant variations in resistance to disinfectants.Among 3 categories of bacterial strains(gram-negative bacteria,gram-positive bacteria and spore-forming bacteria),there were strains within each category that exhibit higher resistance to disinfectants than 2 kinds of biological indicators.Conclusion Sodium hypochlorite and hydrogen peroxide exhibit superior bactericidal effects compared to benzalkonium bromide.The resistance to disinfectant of bacteria is species-specific and cannot be predicted solely by gram classification.The screening scope for biological indicators should be expanded based on actual needs.
作者
刘娜
王亚萍
任秀
赵帅
王学硕
窦越
崔生辉
李景云
LIU Na;WANG Ya-Ping;REN Xiu;ZHAO Shuai;WANG Xue-Shuo;DOU Yue;CUI Sheng-Hui;LI Jing-Yun(National Institute for Food and Drug Control,Beijing 100050,China)
出处
《食品安全质量检测学报》
2025年第14期285-290,共6页
Journal of Food Safety and Quality
基金
国家重点研发计划项目(2022YFF1103100)。
关键词
消毒剂
指示微生物
种属
抗性
差异
disinfectant
biological indicators
species
resistance
difference
作者简介
第一作者:刘娜(1988-),女,硕士,助理研究员,主要研究方向为食品安全检测。E-mail:xinyiliuna@163.com;通信作者:崔生辉(1972-),男,博士,研究员,主要研究方向为食品安全检测。E-mail:cuishenghui@aliyun.com;通信作者:李景云(1972-),男,主任技师,主要研究方向为食品安全检测。E-mail:lijingyun@nifdc.org.cn。
