摘要
目的 探讨BOLA家族成员2(BOLA2)在结直肠癌组织中的表达及与其临床意义。方法 基于UALCAN网站分析TCGA数据库中结直肠癌组织的BOLA2表达情况。利用免疫组化技术和实时定量PCR检测结直肠癌组织和癌旁组织中BOLA2的表达水平,分析BOLA2表达与结直肠癌临床病理特征及预后的关系,采用Cox比例风险回归模型进行多因素生存分析。结果 在线分析结果显示286例结肠癌组织的BOLA2水平[中位数(四分位数)]为115.14(97.157~145.736),高于41例正常组织的80.482(75.660~93.820),166例直肠癌组织的BOLA2水平[中位数(四分位数)]为158.980(104.723~241.041),高于10例正常组织的76.605(71.729~80.835),差异均有统计学意义(P<0.001)。80例结直肠癌组织的BOLA2积分光密度值、阳性率和mRNA水平分别为2.262±0.357、42.5%(34/80)和4.512±1.974,均高于癌旁组织的1.001±0.464、26.25%(21/80)和1.005±0.772,差异均有统计学意义(P<0.001)。Kaplan-Meier生存曲线显示,BOLA2阴性表达者的中位总生存期未达,长于阳性表达者的34.5个月(HR=4.588, 95%CI:2.019~10.426,P<0.001);进一步多因素分析发现BOLA2阳性表达是结直肠癌患者不良预后的危险因素(HR=4.197,95%CI:2.144~8.216,P=0.007)。结论 BOLA2高表达参与了结直肠癌的恶性进展和不良预后,该因子有望成为结直肠癌的潜在诊治靶点。
Objective To investigate the expression and clinical significance of BolA family member 2(BOLA2)in colorectal cancer tissues.Methods The expression of BOLA2 in colorectal cancer tissues from the TCGA database was analyzed based on the UALCAN website.Immunohistochemistry and real-time quantitative PCR were used to detect the expression levels of BOLA2 in colorectal cancer tissues and adjacent normal tissues.The relationship between BOLA2 expression and the clinicopathological features as well as the prognosis of colorectal cancer was analyzed.Multivariate analysis was performed using the Cox proportional hazards regression model.Results Online analysis results showed that the BOLA2 level[median(IQR)]in 286 colon cancer tissues was 115.14(97.157-145.736),significantly higher than 80.482(75.660-93.820)in 41 normal tissues.Similarly,the BOLA2 level in 166 rectal cancer tissues was 158.980(104.723-241.041),significantly higher than 76.605(71.729-80.835)in 10 normal tissues,with both differences being statistically significant(P<0.001).In 80 colorectal cancer tissues,the integrated optical density value,positive rate and mRNA level of BOLA2 were 2.262±0.357,42.5%(34/80)and 4.512±1.974,respectively,all significantly higher than 1.001±0.464,26.25%(21/80)and 1.005±0.772 in adjacent normal tissues with statistically significant differences(P<0.001).Kaplan-Meier survival curve revealed that the median overall survival of BOLA2-negative patients was not reached,which was longer than 34.5 months of BOLA2-positive patients(HR=4.588,95%CI:2.019-10.426,P<0.001).Further multivariate analysis confirmed that BOLA2-positive expression was an independent risk factor for poor prognosis in colorectal cancer patients(HR=4.197,95%CI:2.144-8.216,P=0.007).Conclusion High expression of BOLA2 contributes to the malignant progression and poor prognosis of colorectal cancer,suggesting its potential as a therapeutic and diagnostic target for this disease.
作者
胡延伟
张晓伟
连彦军
范现英
HU Yanwei;ZHANG Xiaowei;LIAN Yanjun;FAN Xianying(Department of Gastrointestinal Surgery,Xingtai Central Hospital,Xingtai 054000,China)
出处
《临床肿瘤学杂志》
2025年第4期378-382,共5页
Chinese Clinical Oncology
基金
刑台市科技局课题资助项目(2022ZC188)。
关键词
结直肠癌
BOLA家族成员2
临床意义
预后
Colorectal cancer
BolA family member 2
Clinical significance
Prognosis
作者简介
通讯作者:范现英,E-mail:741510588@qq.com。
