摘要
马铃薯晚疫病数量抗性受微效多基因控制,通过表型评价选择效率低下。为创制马铃薯数量抗性种质资源,本研究选用8个亲本材料杂交构建了9个杂交群体,在基于离体叶片抗性鉴定评价晚疫病抗性的基础上,结合SNP位点检测进行分子辅助选择。基于数量抗性基因PCR产物测序,检测了60个抗、感杂交后代材料8个数量抗性基因的19个单核苷酸多态性(SNP)位点,结果表明StAOS2-SNP691、StAOS2-SNP692、StGP28-SNP794、PLOX1-SNP8344有利等位基因与抗性存在正向关联,杂交后代群体中抗病材料的有利等位基因纯合基因型占比为9.56%,高于后代群体中的感病材料(5.65%),同时高于亲本材料(7.89%),联合8个基因19个SNP标记的系统发育分析可将60份材料聚类为3个抗病分支和3个感病分支。结合表型、育种系谱和数量抗性基因分子标记计算与抗晚疫病相关的育种值,筛选出育种值高且具有有利等位基因纯合基因型的PC2312-3、PC2312-15、PC2313-19等优异单株,为马铃薯数量抗性群体遗传改良提供了材料。
The quantitative resistance to potato late blight is controlled by multiple genes each with limited effectiveness as determined through phenotype evaluation.In order to develop novel potato germplasm with enhanced quantitative resistance,this study deployed eight parental lines to construct nine hybrid populations.Using detached leaf assays for late blight resistance evaluation coupled with molecular marker-assisted selection,we analyzed 60 resistant and susceptible hybrid offspring through PCR sequencing of quantitative resistance genes,identifying 19 single nucleotide polymorphism(SNP)sites across eight resistance genes.These results demonstrated that specific favorable allele(StAOS2-SNP691,StAOS2-SNP692,StGP28-SNP794,PLOX1-SNP8344)showed positive correlations with late blight resistance.The proportion of favorable homozygous allele in resistance offspring(9.56%)significantly exceeded that in susceptible offspring(5.65%)and parental materials(7.89%).Phylogenetic analysis based 19 SNP markers from eight genes clustered the 60 materials into three resistant and three susceptible branches.By integrating phenotypic data,breeding pedigree and resistance genes markers,we calculated breeding values for late blight resistance and identified superior genotypes(PC2312-3,PC2312-15,and PC2313-19)exhibiting both high breeding values and favorable SNP homozygous allele,therefore providing valuable genetic materials for improving potato resistance.
作者
张朝辉
李永杰
白成娇
卢丽丽
包丽仙
赵志坚
白建明
杨静
潘哲超
ZHANG Chaohui;LI Yongjie;BAI Chengjiao;LU Lili;BAO Lixian;ZHAO Zhijian;BAI Jianming;YANG Jing;PAN Zhechao(Industrial Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205;College of Plant Protection,Yunnan Agricultural University,Kunming 650000)
出处
《植物遗传资源学报》
北大核心
2025年第6期1215-1228,共14页
Journal of Plant Genetic Resources
基金
云南省农业联合专项重点基金(202101BD070001-022)
国家自然科学基金(32260505)
云南省后备人才项目(202305AC160032)
云南种子种业联合试验室项目(202205AR070001)
国家马铃薯产业技术体系(CARS-09-P03)。
关键词
马铃薯
晚疫病
数量抗性
SNP位点
育种值
potato
late blight
quantitative resistance
SNP loci
breeding value
作者简介
第一作者:张朝辉,研究方向为资源利用与植物保护,E-mail:1368140800@qq.com;通信作者:潘哲超,研究方向为马铃薯分子育种,E-mail:pzc@yaas.org.cn。
