摘要
质体甘油醛-3-磷酸脱氢酶(GAPCp,plastid glyceraldehyde-3-phosphate dehydrogenase)在植物生长发育、能量代谢和逆境响应中起重要作用。本研究以栗子香为材料,成功克隆了IbGAPCp1基因的开放阅读框(ORF)序列。亚细胞定位研究表明,IbGAPCp1编码的蛋白质定位于叶绿体中。定量PCR分析显示,IbGAPCp1在甘薯不同组织中均有表达,其中叶片中的表达量最高;随着块根的发育,IbGAPCp1基因的表达逐渐升高,在105 d时达到最高水平。为进一步探索IbGAPCp1基因的调控机制,克隆了长为1940 bp的IbGAPCp1基因启动子序列Pro-IbGAPCp1。顺式作用元件预测结果表明,Pro-IbGAPCp1中含有多个与光响应、激素响应及分生组织表达等相关的元件。干旱和盐胁迫处理下IbGAPCp1基因的表达动态结果显示,IbGAPCp1基因对干旱和盐胁迫均有一定的响应。进一步通过构建诱饵载体并利用酵母单杂交技术筛选出了35个与IbGAPCp1启动子区相互作用的上游调控因子,其中包括乙烯不敏感蛋白2、CCCH型锌指蛋白47及MYB44等。这些因子与植物的生长发育、次生代谢、能量产生及多种逆境响应相关。本研究为深入探讨IbGAPCp1在甘薯非生物胁迫中的功能及作用机制提供了理论基础。
Plastid glyceraldehyde-3-phosphate dehydrogenase(GAPCp)plays an important role in plant growth and development,energy metabolism,and stress response.In this study,we cloned the open reading frame(ORF)sequence of the IbGAPCp1 gene from sweetpotato variety'Lizixiang'.Subcellular localization studies showed that the IbGAPCp1 protein is targeted to the chloroplasts.Quantitative real-time PCR analysis showed that IbGAPCp1 is ubiquitously expressed across sweetpotato tissues,with the highest transcripts detected in leaves.The expression of IbGAPCp1 gene increased gradually with the development of sweetpotato tuberous root and reaching its peak at 105 days.In order to further explore the regulatory mechanism of IbGAPCp1 gene,we cloned a 1940 bp sequence of the IbGAPCp1 gene promoter,designated as Pro-IbGAPCp1.The prediction of cis-acting elements showed that Pro-IbGAPCp1 contains several elements related to photoresponse,hormone response and meristem expression.Expression profiling under drought and salt stress showed that IbGAPCp1 is responsive to both drought and salt stresses.Furthermore,yeast one-hybrid screening identified 35 upstream transcriptional regulators interacting with the promoter region of IbGAPCp1,including ethylene-insensitive protein 2,zinc finger CCCH domain-containing protein 47 and MYB44,factors that are known to modulate plant growth and development,secondary metabolism,energy production,and stress responses.This study provides a theoretical basis for future elucidating the functional role of IbGAPCp1 in sweetpotato abiotic stress tolerance.
作者
曹艳东
白楠楠
罗状
郑亚婷
赵彩良
唐锐敏
武小平
贾峥嵘
吴宇浩
陈伟
贺立恒
贾小云
CAO Yandong;BAI Nannan;LUO Zhuang;ZHENG Yating;ZHAO Cailiang;TANG Ruimin;WU Xiaoping;JIA Zhengrong;WU Yuhao;CHEN Wei;HE Liheng;JIA Xiaoyun(College of Life Sciences,Shanxi Agricultural University/Shanxi Engineering Research Center for Genetics and Metabolism of Special Crops,Taigu 030801;College of Agriculture,Shanxi Agricultural University/Shanxi Engineering Research Center for Genetics and Metabolism of Special Crops,Taigu 030801;Maize Research Institute,Shanxi Agricultural University,Xinzhou 034000;Sorghum Research Institute,Shanxi Agricultural University,Jinzhong 030600;Institute of Cotton Research,Shanxi Agricultural University,Yuncheng 044000)
出处
《植物遗传资源学报》
北大核心
2025年第6期1119-1131,共13页
Journal of Plant Genetic Resources
基金
山西省研究生科研创新项目(2023KY321)
中央引导地方科技发展资金项目(YDZJSX2024B007)
山西农业大学科技创新提升工程(CXGC2023051,CXGC2023074)
山西农业大学杂粮研究院项目(Z120220502,ZLZD2404)
山西省回国留学人员科研资助项目(2021-074)
山西省基础研究计划(202403021212098)。
关键词
甘薯
质体甘油醛-3-磷酸脱氢酶
酵母单杂交
非生物胁迫
sweetpotato
plastid glyceraldehyde-3-phosphate dehydrogenase
yeast one-hybrid
abiotic stress
作者简介
第一作者:曹艳东,研究方向为植物重要功能基因的挖掘与应用,E-mail:kkgxb592260@163.com;通信作者:贾小云,研究方向为薯类作物遗传育种,E-mail:jiaxiaoyun@sxau.edu.cn。
