摘要
该研究以机制和脚踩高温大曲为研究对象,采用高通量测序技术对其蕴含的真菌菌群进行了对比分析。通过α多样性分析可知,与脚踩曲相比,机制曲所蕴含真菌菌群的丰富度和多样性均非常显著偏低(P<0.01);通过置换多因素方差分析可知,两类高温大曲样品真菌菌群的构成均存在非常显著差异(P<0.01);通过对真菌菌群进行解析可知,机制曲中酵母共生菌属(Symbiotaphrina)、干霉菌属(Xeromyces)和丝衣霉属(Byssochlamys)的含量显著偏高(P<0.05),它们在机制曲中相对含量分别为9.51%、3.52%和0.90%,在脚踩曲中相对含量分别为0.02%、0.02%和0.00%;而脚踩曲中罗森氏菌属(Rasamsonia)和嗜热子囊菌属(Thermoascus)的含量显著偏高(P<0.05),它们在机制曲中相对含量分别为0.01%和5.67%,在脚踩曲中相对含量分别为1.47%和27.82%;通过操作分类单元(operational taxonomic units,OTU)分析可知,有1569和3727个OTU分别只在机制曲或脚踩曲真菌菌群中存在,分别仅占总序列数的0.86%和1.58%,而有8305个OTU同时存在于两类高温大曲的若干个样品中,占总序列数的97.56%。通过共现网络分析可知,机制曲和脚踩曲共现网络的平均度分别为4.034和2.718。由此可见,机制曲和脚踩曲中真菌的菌群结构和菌属间的作用关系均存在差异,但菌群的差异主要体现在含量较低的物种上,同时较之脚踩曲,机制曲中真菌属间的稳定性更高。
This study focused on the mechanical and artificial high-temperature Daqu and used high-throughput sequencing technology to compare and analyze the fungal communities contained in it.According to the analysis ofαdiversity,it could be concluded that compared with artificial high-temperature Daqu,the richness and diversity of fungal communities contained in the mechanical high-temperature Daqu were significantly lower(P<0.01).Through permutation multivariate analysis of variance,it could be concluded that there were significant differences in the composition of fungal communities between the two types of high-temperature Daqu samples(P<0.01).By analyzing the fungal community,it was found that the contents of Symbiotaphrina,Xeromyces,and Byssochlamys in the mechanical hightemperature Daqu were significantly higher(P<0.05),with relative contents of 9.51%,3.52%,and 0.90%in the mechanical hightemperature Daqu,and 0.02%,0.02%,and 0.00%in the artificial high-temperature Daqu,respectively.However,the content of Rasamsonia and Thermoascus genera in the artificial high-temperature Daqu was significantly higher(P<0.05),with relative contents of 0.01%and 5.67%in the mechanical high-temperature Daqu,and 1.47%and 27.82%in the artificial high-temperature Daqu,respectively.Through the analysis of operational taxonomic units(OTUs),it was found that 1569 and 3727 OTUs were only present in the fungal communities of the mechanical and artificial high-temperature Daqu,respectively,accounting for only 0.86%and 1.58%of the total sequence number,while 8305 OTUs were simultaneously present in several samples of the two types of high-temperature Daqu,accounting for 97.56%of the total sequence number.Through co-occurrence network analysis,it could be concluded that the average degrees of the co-occurrence networks for mechanical and artificial high-temperature Daqu were 4.034 and 2.718,respectively.From this,it could be seen that there were differences in the fungal community structure and the interaction relationship between fungal genera in the mechanical and artificial high-temperature Daqu,but the differences in fungal communities were mainly reflected in species with lower content.At the same time,compared with the artificial high-temperature Daqu,the stability between fungal genera in the mechanism was higher.
作者
葛东颖
蔡文超
余培荣
陈炜
单春会
王玉荣
郭壮
GE Dongying;CAI Wenchao;YU Peirong;CHEN Wei;SHAN Chunhui;WANG Yurong;GUO Zhuang(Brewing Technology Industrial College,Hubei University of Arts and Sciences,Xiangyang 441053,China;Hubei Provincial Engineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,China;The Food College of Shihezi University,Shihezi 832000,China;Xinjiang Wuwu Liquor Industry Co.Ltd.,Huyanghe 830099,China)
出处
《食品与发酵工业》
北大核心
2025年第14期313-319,共7页
Food and Fermentation Industries
基金
新疆生产建设兵团第七师胡杨河市“揭榜挂帅”项目(QS2024011)。
作者简介
第一作者:葛东颖,硕士研究生;通信作者:郭壮,教授,E-mail:guozhuang1984@163.com。
