摘要
目的采用UPLC-Q-Exactive-Orbitrap MS/MS技术,对白豆蔻水提物主要化学成分及大鼠口服入血成分进行分析。方法采用ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm)色谱柱;以A(去离子水,含0.1%甲酸)和B(乙腈,含0.1%甲酸)为流动相,梯度洗脱,柱温:35℃;进样体积:10μL;流速:0.2 mL·min^(-1)。采用电喷雾离子源(ESI),Full MS/dd-MS2模式检测。结果从白豆蔻水提物中共鉴定70种成分,包括有机酸类、黄酮类、氨基酸类、生物碱类、内酯类等。含药血清样本中分析19种原型成分,包括青蒿酸、壬二酸等10种有机酸类,异土木香内酯、去氢木香内酯2种内酯类,甜菜碱、黄嘌呤2种生物碱类,酪氨酸、色氨酸等3种氨基酸。结论本研究首次阐明了白豆蔻水提物化学成分及其口服入血成分,为其进一步研究与开发提供科学的理论依据。
OBJECTIVE To analyze the main components of Baidoukou aqueous extract and the components absorbed into blood of rats after oral administration by UPLC-Q-Exactive-Orbitrap MS/MS technology.METHODS Using ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm)column;using A(deionized water,containing 0.1% formic acid)and B(acetonitrile,containing 0.1% formic acid)as mobile phases with gradient elution,column temperature was 35℃,while injection volume was 10μL,and flow rate was 0.2 mL·min^(-1).Using electrospray ionization source(ESI),detect with Full MS/dd-MS2 mode.RESULTS A total of 70 components were identified from the aqueous extract of Baidoukou,including organic acids,flavonoids,amino acids,alkaloids,and lactones.Nineteen prototype components were identified in the drug-containing serum samples,including 10 organic acids such as artemisinic acid and azelaic acid,2 lactones such as isoalantolactone and dehydrocostus lactone,2 alkaloids such as betaine and xanthine,3 amino acids such as L-tyrosine and L-tryptophan.CONCLUSION This study sclarified the chemical composition of Baidoukou aqueous extract and its oral blood components for the first time,providing a theoretical basis for its further research and development.
作者
巴达拉胡
吴杰斯
包白音木其尔
刘小伟
敖道夫
BA Dalahu;WU Jiesi;BAO Baiyinmuqi’er;LIU Xiaowei;AO Daofu(Affiliated Hospital of Inner Mongolia Minzu University,Tongliao 028000,China)
出处
《中国现代应用药学》
北大核心
2025年第9期1492-1500,共9页
Chinese Journal of Modern Applied Pharmacy
基金
内蒙古自治区自然科学基金项目(2021LHMS08030)。
作者简介
巴达拉胡,男,硕士,副主任医师,E-mail:nmfoot@126.com;通信作者:敖道夫,女,硕士,副主任蒙药师,E-mail:15004986906@163.com。
