摘要
[目的]探究低共熔溶剂对花椒叶总黄酮提取率的影响,并分析其抗氧化活性。[方法]以花椒叶为原料,总黄酮提取率为指标,采用超声辅助进行提取,筛选出最佳低共熔溶剂体系,并测定花椒叶总黄酮化合物对羟自由基和DPPH自由基的清除能力。[结果]采用氯化胆碱—乙二醇(n_(氯化胆碱)∶n_(乙二醇)为1∶3)为低共熔溶剂,当溶剂含水量为22%,料液比(m_(花椒粉末)∶V_(溶剂))为1∶47 (g/m L),超声时间为15 min时,花椒叶总黄酮提取率最大;花椒叶总黄酮对羟自由基的清除率最高为(63.89±0.26)%,对DPPH自由基的清除率可达(89.01±0.51)%。[结论]低共熔溶剂提取花椒叶总黄酮效果佳,且该花椒叶总黄酮的抗氧化能力较好。
[Objective]To investigate the effect of deep eutectic solvent(DES)on the extraction rate of total flavonoids from Zanthoxylum bungeanum Maxim.leaves and analyze their antioxidant activity.[Methods]Using Z.bungeanum leaves as raw material and total flavonoid extraction rate as the evaluation index,this study performs ultrasound-assisted extraction to screen the optimal DES system.The scavenging capacities of the extracted total flavonoids against hydroxyl radicals(·OH)and DPPH radicals are determined.[Results]The highest extraction rate of total flavonoids is achieved using a choline chloride-ethylene glycol DES system(ncholine chloride∶nethylene glycol=1∶3)with 22%water content,a solid-to-liquid ratio(mZ.bungeanum powder∶Vsolvent)of 1∶47(g/mL),and ultrasonic time of 15 min.The total flavonoids from Z.bungeanum leaves exhibit a maximum hydroxyl radical scavenging rate of(63.89±0.26)%and a DPPH radical scavenging rate as high as(89.01±0.51)%.[Conclusion]DES demonstrates excellent efficiency in extracting total flavonoids from Z.bungeanum leaves,and the extracted total flavonoids possess strong antioxidant activity.
作者
孙伟
谷清义
翟冰倩
叶润
蔡静
乔新荣
SUN Wei;GU Qingyi;ZHAI Bingqian;YE Run;CAI Jing;QIAO Xinrong(School of Pharmacy,Xinyang Agriculture and Forestry University,Xinyang,Henan 464000,China)
出处
《食品与机械》
北大核心
2025年第7期199-206,共8页
Food and Machinery
基金
河南省重点研发专项(编号:241111311400)
信阳农林学院大别山道地中药资源与系列产品开发研究科技创新团队(编号:XNKJTD-009)
河南省科技公关项目(编号:242102230094)。
关键词
低共熔溶剂
花椒叶
总黄酮
抗氧化
deep eutectic solvent
Zanthoxylum bungeanum Maxim.leaf
total flavonoids
antioxidant
作者简介
通信作者:叶润(1988-),男,信阳农林学院讲师,硕士。E-mail:yerun@126.com。
