摘要
斜纹夜蛾Spodoptera litura作为世界性分布的农林业害虫,具有繁殖力强、爆发快等特点,大发生时能给农林牧业生产和生态环境维持造成巨大的损失。蝉花虫草Cordyceps chanhua是我国重要的虫生真菌资源,其菌丝体及子实体内存在多种次生代谢产物(如白僵菌素、恩镰孢菌素等),对多种鳞翅目等害虫具有较强的杀虫活性。本研究探索蝉花对斜纹夜蛾的致病作用,进一步明确蝉花对斜纹夜蛾致病相关的毒素和致病基因,为蝉花对斜纹夜蛾致病机理研究提供参考。采用蝉花孢子悬浮液浸渍室内生物测定法,检测蝉花对斜纹夜蛾的致病力。敲除蝉花白僵菌素合成相关的组蛋白去乙酰化酶基因PP558224,验证蝉花白僵菌素产量与致病力的关系。研究表明,蝉花孢子悬浮液对斜纹夜蛾二龄幼虫具有一定致病力,敲除PP558224基因后蝉花白僵菌素含量显著降低,与野生型菌株比较,敲除菌株对斜纹夜蛾致病力显著降低,推测蝉花白僵菌素对斜纹夜蛾致病性具有重要作用,且白僵菌素含量与其致病力呈正相关。
As a globally distributed agroforestry pest,twill armyworm Spodoptera litura exhibits strong fecundity and rapid outbreak potential.Its occurrence can lead to significant losses in agroforestry production and the maintenance of ecological environments.Cordyceps chanhua mycelium and fruiting bodies contain a diverse array of secondary metabolites,including beauvericin(BEA)and enniatins(ENNs),which exhibit potent insecticidal properties against various lepidopteran species and other pests.This study explored the pathogenic effect of C.chanhua on S.litura,and further clarified the toxins and pathogenic genes related to the pathogenesis of C.chanhua,so as to provide a reference for the study of the mechanism responsible for pathogenicity of C.chanhua against S.litura.The laboratory bioassay method of C.chanhua spore suspension immersion was used to detect the pathogenicity of C.chanhua against S.litura.PP558224 histone deacetylase gene related to the synthesis of beauvericin in C.chanhua was knocked out to verify the relationship between the production of beauvericin in C.chanhua and its pathogenicity.The research shows that the C.chanhua spore suspension has a certain pathogenic effect on the second-instar larvae of S.litura.After knocking out the PP558224 gene,the content of beauvericin in C.chanhua was significantly reduced.Compared with the wild-type strain,the knock-out strains of C.chanhua showed remarkable reduction of pathogenic effect on the second-instar larvae of S.litura,indicating that the beauvericin plays an important role in the pathogenicity of C.chanhua,and the content of beauvericin is positively correlated with the pathogenicity.
作者
俞孔见
胡加付
柴一秋
蔡瑞杭
YU Kongjian;HU Jiafu;CHAI Yiqiu;CAI Ruihang(College of Forestry and Biotechnology,Zhejiang Agricultural&Forestry University,Hangzhou 311300,Zhejiang,China;Zhejiang Institute of Subtropical Crops,Key Laboratory of Entomogenous Fungus Resources Research and Development of Wenzhou,Wenzhou 325005,Zhejiang,China)
出处
《菌物学报》
北大核心
2025年第6期108-119,共12页
Mycosystema
基金
浙江省重点研发项目(202002026)
温州市科学技术局揭榜挂帅项目(ZN2024001)。
关键词
斜纹夜蛾
蝉花虫草
敲除突变
白僵菌素
致病力
Spodoptera litura
Cordyceps chanhua
knockout mutation
beauvericin
pathogenicity
作者简介
Corresponding authors:HU Jiafu,E-mail:hujiafu2000@163.com;Corresponding authors:CHAI Yiqiu,E-mail:chaiyiqiu@qq.com。
