摘要
目的分析肿瘤坏死因子样细胞弱凋亡因子(tumor necrosis factor-like cell weak apoptosis factor,TWEAK)在诱导痰中的表达以及与哮喘严重程度的关联性。方法收集2022年3月至2022年9月我院收治的50例哮喘患者为对象,使用酶联免疫吸附法测定诱导痰TWEAK值。根据TWEAK中位值分为TWEAK高表达组(≥0.70 ng/ml)和TWEAK低表达组(<0.70 ng/ml),每组25例。使用Spearman分析TWEAK与肺功能指标、炎症指标和M1型巨噬细胞的相关性。结果TWEAK高表达组干扰素(interferon,IFN)-γ0.80(0.24,2.07)pg/ml低于低表达组3.07(0.61,17.31)pg/ml(P<0.05);高表达组白细胞介素(interleukin,IL)-17A 0.35(0.07,1.22)pg/ml、CD45^(+)/CD68^(+)巨噬细胞%5.98(3.36,8.02)、M1型(CD11c^(+)/CD86^(+))巨噬细胞%5.44(1.59,8.44)高于TWEAK低表达组0.11(0.03,0.23)pg/ml、3.03(1.60,5.75)、2.45(1.52,4.47)(P<0.05)。经Spearman分析,诱导痰TWEAK与1秒用力呼气容积(FEV_(1))占预计值的百分比(r_(s)=-0.476)、FEV_(1)/用力肺活量(r_(s)=-0.394)、诱导痰IFN-γ(r_(s)=-0.346)呈显著负相关(P<0.05)。诱导痰TWEAK与肿瘤细胞坏死因子(tumor necrosis factor,TNF)-α(r_(s)=0.315)、IL-6(r_(s)=0.286)、IL-17A(r_(s)=0.320)、M1型(CD11c^(+)/CD86^(+))巨噬细胞%(r_(s)=0.470)、中性粒细胞%(r_(s)=0.293)以及外周血中性粒细胞%(r_(s)=0.351)呈显著正相关(P<0.05)。有过敏家族史的患者诱导痰TWEAK表达显著低于无过敏家族史(P<0.05)。结论诱导痰TWEAK过表达与哮喘患者炎症进展和疾病发作存在相关性,诱导痰中TWEAK可能是哮喘严重程度的标志物。
Objective To investigate the expression of tumor necrosis factor-like cell weak apoptosis factor(TWEAK)in induced sputum and its association with asthma severity.Methods A total of 50 asthma patients were recruited from March 2022 to September 2022,and induced TWEAK in sputum were determined using enzyme-linked immunosorbent assay.According to the TWEAK value,asthma patients were divided into TWEAK high expression group(≥0.70 ng/ml)and TWEAK low expression group(<0.70 ng/ml),with 25 cases in each group.Spearman was used to analyze the correlation between TWEAK and lung function indexes,inflammation markers and M1 macrophages.Results The interferon(IFN)-γin the TWEAK high expression group were significantly lower than those in the low expression group(P<0.05),and the percentages of interleukin(IL)-17A,CD45^(+)/CD68^(+)macrophages%,M1 type(CD11c^(+)/CD86^(+))macrophages%in the TWEAK high expression group were significantly higher than those in the low expression group(P<0.05).According to Spearman analysis,induced sputum TWEAK was significantly negatively correlated with the percentage of forced expiratory volume(FEV_(1))in 1 second(r_(s)=-0.476),FEV_(1)/forced vital capacity(r_(s)=-0.394),and induced sputum IFN-γ(r_(s)=-0.346)(P<0.05).The induced sputum TWEAK was significantly positively correlated with tumor cell necrosis factor-α(r_(s)=0.315),IL-6(r_(s)=0.286),IL-17A(r_(s)=0.320),M1 type(CD11c^(+)/CD86^(+))macrophage%(r_(s)=0.470),neutrophil%(r_(s)=0.293)and peripheral blood neutrophil%(r_(s)=0.351)(P<0.05).The expression of induced sputum TWEAK in patients with a family history of allergies was significantly lower than that in patients without a family history of allergies(P<0.05).Conclusion There was a significant correlation between induced sputum TWEAK overexpression and inflammation progression and disease flare-up in asthma patients,and induced TWEAK in sputum may be a marker of asthma severity.
作者
郭依丹
王军
朱祥
吉泽
王蓓娟
Guo Yidan;Wang Jun;Zhu Xiang;Ji Ze;Wang Beijuan(Department of Respiratory and Critical Care Medicine,Suzhou Jiulong Hospital,Shanghai Jiao Tong University School of Medicine,Suzhou 215127,China)
出处
《中华肺部疾病杂志(电子版)》
2025年第3期375-379,共5页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
苏州市卫生健康委科研课题(项目编号:YQWS202203)。
作者简介
通信作者:王蓓娟,Email:lotus7377@163.com。
