摘要
目的通过全自动血细胞处理系统ACP215自动化制备洗涤血小板,并对洗涤后的血小板体外质量做出评估。方法基于ACP215洗涤程序,应用改良后一次性耗材,及配置的血小板洗涤液、悬浮液,对单袋血小板进行全自动洗涤制备。对洗涤前后血小板容量、血小板含量、血浆蛋白含量、血小板分布宽度(PDW)、平均血小板体积(MPV)、pH值进行比较,并与传统手工洗涤方式作对比。机制洗涤血小板在保存24 h后,进行血小板聚集实验、血栓弹力图MA值读取和亚细胞结构光电成像。结果机制洗涤血小板基础检测符合相关质量标准,回收率为(87.55±2.54)%,血浆蛋白清除率为(95.84±0.69)%,均显著高于传统手工法(Z=-13.633,P<0.01;Z=-12.039,P<0.01)。机制洗涤前后血小板的最大聚集率和聚集时间均无统计学差异(t=1.273,P=0.218;t=0.770,P=0.451),MA值略减少(t=2.236,P=0.038),机制洗涤前和洗涤后血小板的vWF及微管结构无统计学差异(χ^(2)=2.257,P=0.133;χ^(2)=0.523,P=0.469)。结论使用全自动血细胞处理系统ACP215可标准化、自动化制备洗涤血小板且符合质量要求,在保存24 h后洗涤血小板体外功能良好。
Objective To prepare washed platelets by automatic blood cell treatment system ACP215 and evaluate the in vitro quality of the platelets after washing.Methods Based on the ACP215 washing program,the improved disposable consumables,as well as the prepared platelet washing solution and suspension,were applied to automatically wash and prepare single bags of platelets.The platelet volume,platelet content,plasma protein content,PDW,MPV and PH before and after washing were compared,and compared with the traditional manual washing method.After storage for 24 hours,platelet aggregation test,thromboelastography MA evaluation and subcellular structure photoimaging were performed.Results The recovery rate was(87.55±2.54)%and plasma protein clearance was(95.84±0.69)%,which were significantly higher than the conventional manual method(Z=-13.633,P<0.01;Z=-12.039,P<0.01).There was no statistical difference in maximum aggregation and aggregation time of platelets before and after washing(t=1.273,P=0.218;t=0.770,P=0.451).MA value was slightly reduced(t=2.236,P=0.038).There was no statistical difference in subcellular structure before and after washing(χ^(2)=2.257,P=0.133;χ^(2)=0.523,P=0.469).ConclusionUtilization of automatic blood cell treatment system ACP215 can standardize and automatically prepare washing platelets which meet the quality requirements.The platelets has good in vitro function after washing and 24 hours of storage.
作者
郑雪
戴丽花
马梦迪
任明
Zheng Xue;Dai Lihua;Ma Mengdi(Department of Composition Preparation,Wuhan Blood Center,Wuhan 430030,China)
出处
《华中科技大学学报(医学版)》
北大核心
2025年第3期395-400,共6页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
武汉市医学科学研究项目(No.WX23B38)。
作者简介
郑雪,女,1982年生,副主任技师,医学硕士,E-mail:29780127@qq.com;通信作者:任明,E-mail:84347756@qq.com。
