摘要
旨在探究奶牛外周血浆中激素、氧化应激指标及微量元素等因子与早期妊娠的相关性,为奶牛早期妊娠诊断提供参考。本研究选取宁夏某奶牛场120头健康经产荷斯坦奶牛(2~3胎次),体况评分相近,经同期发情处理后,在定时人工授精(timed artificial insemination,TAI)后第7天采集血浆。TAI后18~24 d采用计步法观察返情,第35天采用B超进行妊娠诊断。随后根据诊断结果将奶牛分为妊娠组(n=46)、未妊娠组(n=34)和返情组(n=40)。使用ELISA检测3组血浆样本总抗氧化能力(total antioxidant capacity,T-AOC)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)、过氧化氢酶(catalase,CAT)、皮质醇(cortisol,COR)、褪黑素(melatonin,MT)、前列腺素E2(prostaglandin E2,PGE2)、前列腺素F2α(prostaglandin F2α,PGF2α)、孕酮(progesterone,P4)、雌二醇(estradiol,E2)、铜(copper,Cu)和锌(zinc,Zn)等指标的含量。结果显示:1)120头奶牛中46头妊娠(妊娠组,38.33%)、40头返情(返情组,33.33%)、34头未妊娠(未妊娠组,28.34%);2)妊娠组和未妊娠组奶牛血浆PGE2、COR、T-AOC、GPX4、CAT和Zn的浓度显著高于返情组(P<0.05);未妊娠组奶牛MDA浓度显著高于妊娠组(P<0.05),其余各组各指标间差异不显著(P>0.05);3)分析各指标浓度与妊娠率之间的关系发现,E2、P4、PGE2、PGF2α、MT、COR、T-AOC、GPX4、CAT、MDA、SOD、Cu和Zn的浓度分别为239.27~292.73 ng·L^(-1)、4.71~6.95 ng·mL^(-1)、767.36~941.70 ng·L^(-1)、386.70~489.05 ng·L^(-1)、1664.34~2271.51 ng·L^(-1)、316.32~382.36 ng·mL^(-1)、0.34~0.39 mmol·L^(-1)、62.80~71.90 mg·mL^(-1)、10.67~17.75 U·mL^(-1)、1.15~1.80 nmol·mL^(-1)、14.20~15.73 U·mL^(-1)、12.00~24.00μmol·L^(-1)和14.71~16.53μmol·L^(-1)时奶牛的妊娠率最高,分别为75%、47%、63%、56%、48%、60%、57%、50%、50%、50%、57%、50%和50%;4)根据ROC曲线计算AUC值,奶牛在TAI后第7天血浆中Zn、COR和PGE2的AUC值较高(AUC>0.6)。综上,TAI后第7天低浓度的T-AOC、CAT、GPX4、COR和PGE2和Zn与奶牛返情相关,低浓度的MDA与奶牛妊娠相关,且其不同浓度对应了奶牛妊娠率的差异,Zn、COR和PGE2可作为返情奶牛早期妊娠诊断的候选生物标志物。
This experiment aimed to explore the correlations between hormones,oxidative stress indicators,trace elements,and other factors in the peripheral plasma and pregnancy status of cows during early pregnancy,providing references for the early pregnancy diagnosis of cows.In this experiment,120 healthy multiparous Holstein cows(2-3 parities)with similar body condition scores were selected from a dairy farm in Ningxia.Following estrus synchronization treatment,plasma samples were collected on the 7 th day after timed artificial insemination(TAI).The recurrence of estrus was observed using the step-counting method from 18 to 24 days post-TAI,and pregnancy diagnosis was performed via B-ultrasound on the 35 th day.Based on the diagnosis results,the cows were categorized into 3 groups:pregnant(n=46),non-pregnant(n=34),and return(n=40).ELISA was utilized to measure the levels of total antioxidant capacity(T-AOC),malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase 4(GPX4),catalase(CAT),cortisol(COR),melatonin(MT),prostaglandin E2(PGE2),prostaglandin F2α(PGF2α),progesterone(P4),estradiol(E2),copper(Cu),and zinc(Zn)in plasma samples from the 3 groups.The results indicated that:1)Among the 120 cows,46 were pregnant(pregnancy group,38.33%),40 returned to estrus(the returns group,33.33%),and 34 were not pregnant(non-pregnancy group,28.34%);2)The concentrations of plasma PGE2,COR,T-AOC,GPX4,CAT,and Zn in pregnant cows and non-pregnant cows were significantly higher than those in the returns group(P<0.05),the MDA concentration in non-pregnant cows was significantly higher than that in pregnant cows(P<0.05),while no significant differences were observed in other indices among the groups(P>0.05);3)Analyzing the relationship between the concentrations of each index and pregnancy rate,it was found that when the concentrations of E2,P4,PGE2,PGF2α,MT,COR,T-AOC,GPX4,CAT,MDA,SOD,Cu and Zn were 239.27-292.73 ng·L^(-1),4.71-6.95 ng·mL^(-1),767.36-941.70 ng·L^(-1),386.70-489.05 ng·L^(-1),1664.34-2271.51 ng·L^(-1),316.32-382.36 ng·mL^(-1),0.34-0.39 mmol·L^(-1),62.80-71.90 mg·mL^(-1),10.67-17.75 U·mL^(-1),1.15-1.80 nmol·mL^(-1),14.20-15.73 U·mL^(-1),12.00-24.00μmol·L^(-1) and 14.71-16.53μmol·L^(-1),respectively,the pregnancy rate of cows was the highest,which were 75%,47%,63%,56%,48%,60%,57%,50%,50%,50%,57%,50%and 50%,respectively;4)The ROC curve analysis indicated that the AUC values for Zn,COR,and PGE2 in the plasma of cows on the 7 th day after TAI were relatively high(AUC>0.6).In conclusion,low concentrations of T-AOC,CAT,GPX4,COR,PGE2,and Zn are associated with recurrence of estrus of dairy cows,while low concentrations of MDA are linked to pregnancy on the 7 th after TAI.The varying concentrations of these biomarkers correspond to differences in the pregnancy rates of dairy cows.Zn,COR,and PGE2 could serve as candidate biomarkers for early pregnancy diagnosis in estrus dairy cows.
作者
付予
杨卓
郑浩
孙国瀚
沈文娟
韩小红
陶金忠
FU Yu;YANG Zhuo;ZHENG Hao;SUN Guohan;SHEN Wenjuan;HAN Xiaohong;TAO Jinzhong(School of Animal Science and Technology,Ningxia University,Yinchuan 750021,China)
出处
《畜牧兽医学报》
北大核心
2025年第6期2790-2800,共11页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
宁夏回族自治区重点研发项目(2022BBF03022)。
关键词
奶牛
血浆
妊娠诊断
妊娠率
dairy cow
plasma
pregnancy diagnosis
pregnancy rate
作者简介
付予(2000-),女,河南新乡人,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:fuyu771202@163.com;通信作者:陶金忠,主要从事动物繁殖技术和繁殖障碍的研究,E-mail:tao_jz@nxu.edu.cn。
