摘要
杆状病毒是一类具有双链、环状、超螺旋DNA结构的病毒,其复制、转录以及子代病毒的组装都发生在细胞核内,而由于细胞核膜这一天然屏障的存在,分子量大于40~60 kDa的大分子物质无法直接穿透核膜,因此,病毒进化出了不同的策略来实现病毒蛋白的跨核膜转运。本文综述了杆状病毒编码蛋白的核转运途径,主要有杆状病毒衍生出的依赖核定位信号(nuclear localization signal, NLS)的经典核转运途径和不依赖NLS的非经典核转运途径。此外,病毒还会通过蛋白质修饰、改变宿主结构等方式调控入核途径,从而实现病毒蛋白的核转运。
Baculovirus is a type of virus with a double-stranded,circular,and superhelical DNA structure.The replication and transcription of viral genes,as well as the assembly of progeny virions,occur in the nucleus of the cell.However,owing to the natural barrier of the cell nuclear membrane,macromolecular substances larger than 40-60 kDa cannot directly penetrate the nuclear membrane.Therefore,viruses have evolved different strategies to achieve transmembrane transport of viral proteins.The classical nuclear transport pathways that rely on nuclear localization signal(NLS)and non-classical nuclear transport pathways that do not rely on NLS derived from baculovirus were summarized in this review.Moreover,during the nuclear transport process,viruses regulate the nuclear pathway through protein modifications,host structure changes,and other strategies,thereby creating a favorable environment for the nuclear transport of viral proteins.
作者
李佳乐
王星洋
吴小锋
LI Jiale;WANG Xingyang;WU Xiaofeng(College of Animal Sciences,Zhejiang University,Hangzhou 310058,Zhejiang,China)
出处
《浙江大学学报(农业与生命科学版)》
北大核心
2025年第3期366-378,共13页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金项目(32172793)
国家重点研发计划项目“政府间国际科技创新合作专项”(2022YFE0124400)。
关键词
杆状病毒
核定位信号
核转运受体
核孔复合体
蛋白质修饰
baculovirus
nuclear localization signal
nuclear transport receptor
nuclear pore complex
protein modification
作者简介
通信作者:吴小锋(https://orcid.org/0000-0003-3631-7408),Tel:+86-571-88982198,E-mail:wuxiaofeng@zju.edu.cn;第一作者:李佳乐(https://orcid.org/0009-0008-3146-8454),E-mail:1678520459@qq.com。
