摘要
为筛选防治绿豆象Callosobruchus chinensis的植物源高效驱避剂,利用Y型嗅觉仪测试绿豆象对9种非寄主植物挥发物的选择行为反应,通过网笼产卵试验研究挥发物对绿豆象的产卵忌避效果,并评价不同载体对挥发物驱避作用的影响。结果显示:9种非寄主植物挥发物中有5种对绿豆象雌雄成虫有显著驱避作用,分别是100μg/μL丁香酚、500μg/μL 4-烯丙基苯甲醚、500μg/μL4-萜烯醇、50μg/μL茴香烯和500μg/μL柠檬醛,而50μg/μL D-柠檬烯和100μg/μL芳樟醇仅对绿豆象雌成虫有驱避作用,γ-萜品烯和月桂烯对绿豆象雌雄成虫均无驱避作用。4-萜烯醇和4-烯丙基苯甲醚对绿豆象的产卵忌避效果较好,选择性忌避率分别为81.82%和75.26%,非选择性忌避率分别为60.72%和39.94%。橡胶塞作为缓释载体时,4-萜烯醇与4-烯丙基苯甲醚对绿豆象的产卵忌避率为66.31%和58.23%,显著高于其他载体,其中4-烯丙基苯甲醚的产卵忌避持效期可达10 d。表明4-萜烯醇与4-烯丙基苯甲醚均可作为开发绿豆象植物源驱避剂的候选物质。
To screen out highly effective plant-derived repellents for the control of Chinese bean weevil Callosobruchus chinensis,the selective behavioral responses of C.chinensis to nine non-host plant volatiles were tested with Y-type olfactory apparatus.The oviposition repellent effect of volatiles on C.chinensis was studied using egg-laying experiment in net cages and the effects of different carriers on the repellency of the volatiles were evaluated.The results showed that among the nine volatile compounds from non-host plants,five showed significant repellent effects on both male and female adults,namely 100μg/μL eugenol,500μg/μL 4-allylanisole,500μg/μL 4-terpineol,50μg/μL trans-anethole and 500μg/μL citral.50μg/μL D-limonene and 100μg/μL linalool only exhibited repellent effects on female C.chinensis,while,γ-terpinene and myrcene had no deterrent effect on either male or female adults.4-terpineol and 4-allylanisole exhibited strong oviposition deterrent effects on the beetles,with selective deterrence rates of 81.82%and 75.26%respectively,and non-selective deterrence rates of 60.72%and 39.94%respectively.When the rubber plug was used as a carrier,4-terpineol and 4-allylanisole had oviposition deterrence rates of 66.31%and 58.23%against the beetles,which were significantly higher than those of other carriers.The oviposition deterrent effect of 4-allylanisole lasted up to ten days.This indicated that 4-terpineol and 4-allylanisole could be considered as candidate substances for developing plant-based repellents against the C.chinensis.
作者
张苗
武安琪
马晓瑜
樊楠楠
王科辉
张仙红
郑海霞
Zhang Miao;Wu Anqi;Ma Xiaoyu;Fan Nannan;Wang Kehui;Zhang Xianhong;Zheng Haixia(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,Shanxi Province,China;Shanxi Ronghui Farming Agriculture Co.,Ltd.,Yuncheng 044000,Shanxi Province,China)
出处
《植物保护学报》
北大核心
2025年第2期428-436,共9页
Journal of Plant Protection
基金
国家重点研发计划(2021YFD1600603-01)
财政部和农业农村部国家现代农业产业技术体系(CARS-08-G10)
山西省自然科学基金面上项目(202103021224166)。
关键词
绿豆象
非寄主植物挥发物
选择行为
驱避
产卵忌避
缓释载体
Callosobruchus chinensis
non-host plant volatile
choice behavior
repellency
oviposition deterrence
controlled-release carrier
作者简介
通信作者:郑海霞,E-mail:zhenghaixia722@163.com。
