摘要
[目的]研究对比萌发过程中贵州石楠和黑果石楠种子内含物的动态变化以及转录组分析,探索两种种子萌发过程中涉及的发育机制,为选育新品种及其开发提供参考。[方法]以贵州石楠和黑果石楠种子为研究对象,分别对两种石楠种子萌发过程中的营养物质以及内源激素含量进行测定;采用Illumina NovaSeq6000高通量测序技术对两种石楠种子生长阶段进行转录组测序。[结果]1)两种石楠种子的萌发方式为子叶出土型,在萌发过程中,贵州石楠种子的发芽率高于黑果石楠;2)在干种子状态下,贵州石楠还原糖含量高于黑果石楠。在萌发过程中,二者在营养物质变化趋势上基本一致,但总体而言,贵州石楠的营养物质含量始终高于黑果石楠。此外,干种子阶段下,黑果石楠ABA含量高于贵州石楠,而贵州石楠GA3、IAA和MEIAA的含量则高于黑果石楠。整个萌发过程中,双方IAA含量整体呈下降态势,而GA3/ABA与IAA/ABA比值先升后降;3)转录组测序共获得186810条Unigene,其中157309条Unigene被注释到七大功能数据库,有67.94%的Unigene注释至蔷薇科植物。GO富集分析显示,两类石楠种子的萌发过程主要富集于木质素代谢和氧化还原酶活性等方面。然而,在细胞组成中,光合作用仅在贵州石楠中表现出明显富集。KEGG富集结果表明,在两种石楠种子的萌发过程中,与核糖体、植物激素信号传导及MAPK信号路径相关的代谢通路活跃。在整个生长发展过程中,相较于黑果石楠,贵州石楠调控代谢通路基因数量更多,并且调控幼苗期发展的基因表达也更活跃。此外,多家族转录因子如C3H、AP2、bZIP、MYB等在两种石楠种子的萌发生理中均表现出大量表达。[结论]两种石楠种子萌发过程中内含物动态变化及转录组测序结果表明,由于相关基因的差异表达,植物激素信号调控、MAPK信号以及光合作用等与种子萌发相关代谢活动不同,幼苗阶段贵州石楠生长势优于黑果石楠;内含物含量变化和转录组水平揭示贵州石楠种子解除休眠、启动萌发可能比黑果石楠快。
[Objective]The dynamic changes of seed contents and transcriptome analysis of Photinia bodinieri and P.atropurpurea during germination were studied to explore the developmental mechanisms involved in the germination of the two seeds,and to provide references for the selection and development of new varieties.[Methods]Seeds from both varieties were analyzed for nutrient content and endogenous hormone levels during germination.High-throughput sequencing technology using Illumina NovaSeq6000 was employed for transcriptome sequencing at the seed growth stage of P.bodinieri and P.atropurpurea.[Results]1)The germination mode of the two kinds of photinia seeds was cotyledone-unearthed type.During the germination process,the germination rate of P.bodinieri seeds was higher than that of P.atropurpurea.2)In the dry seed state,the reducing sugar content of P.bodinieri was higher than that of P.atropurpurea.In the process of germination,the change trend of nutrients of the two species was basically the same,but in general,the nutrient content of P.bodinieri was always higher than that of P.atropurpurea.In addition,at the dry seed stage,the ABA content of P.atropurpurea was higher than that of P.bodinieri,while the GA3,IAA and MEIAA contents of P.bodinieri were higher than that of P.atropurpurea.During the whole germination process,the IAA content of both sides decreased,while the ratio of GA3/ABA to IAA/ABA increased first and then decreased.3)A total of 186810 unigenes were obtained through transcriptome sequencing.Among these unigenes,157309 received annotations across seven functional databases with approximately 67.94%annotated within Rosaceae family-related categories.GO enrichment analysis indicated that lignin metabolism along with oxidoreductase activity played significant roles during seed germination processes.However,in terms of cell composition,photosynthesis showed significant enrichment only in P.bodinieri.The results of KEGG enrichment showed that metabolic pathways related to ribosome,plant hormone signaling and MAPK signaling pathways were active during the germination of the two seeds.Compared with P.atropurpurea,P.bodinieri had more genes regulating metabolic pathways and more active genes regulating seedling development during the whole growth and development.In addition,multi-family transcription factors such as C3H,AP2,bZIP and MYB were expressed in large quantities in the germination physiology of the two types of Photinea seeds.[Conclusion]According to the dynamic changes of contents during seed germination of P.bodinieri and P.atropurpurea and transcriptome sequencing results,due to the differences in the expression of related genes,regulation of plant hormone signal,MAPK signal,photosynthesis and other metabolic activities related to seed germination,the growth potential of P.bodinieri was better than that of P.atropurpurea at seedling stage.The changes of contents and transcriptome levels indicated that the seeds of P.bodinieri might release dormancy and start germination faster than that of P.atropurpurea.
作者
黄世宇
赵振利
范国强
王东
冀蒙蒙
翟晓巧
HUANG Shi-yu;ZHAO Zhen-li;FAN Guo-qiang;WANG Dong;JI Meng-meng;ZHAI Xiao-qiao(College of Forestry,Henan Agricultural University,Zhengzhou 450046,Henan,China;Henan Qiyeshu Ecological Development Co.,Ltd,ZhuMadian 463400,Henan,China;Henan Forest Research Institute,Zhengzhou 450002,Henan,China)
出处
《林业科学研究》
北大核心
2025年第3期93-102,共10页
Forest Research
基金
北方阔叶乔木良种收集与新品种培育研究(中原学者工作站:ZYGZZ2022090)资助,项目编号:234400510030。
关键词
贵州石楠
黑果石楠
种子
萌发过程
营养物质
内源激素
转录组
Photinia bodinieri
Photinia atropurpurea
Seeds
Germination process
Nutrients
Endogenous hormone
transcriptome
作者简介
通讯作者:翟晓巧,研究员,主要研究方向:林木遗传育种。电话:13939047391。E-mail:User7117@163.com。
