摘要
目的探究壮骨止痛胶囊对绝经后骨质疏松症模型大鼠JNK信号通路及其磷酸化蛋白的影响。方法将大鼠按随机数字表法分为假手术组、空白组、模型组、阳性药物组、壮骨止痛方组,每组10只。模型组、阳性药物组、壮骨止痛方组采用双侧去卵巢法制备绝经后骨质疏松症大鼠模型。阳性药物组灌胃阿仑膦酸钠0.9 mg/kg,壮骨止痛方组灌胃壮骨止痛胶囊1.944 g/kg,空白组、假手术组、模型组灌胃等体积生理盐水,1次/d,共持续13周。取大鼠阴道脱落细胞进行瑞氏染色,采用ELISA法测定血清网膜素-1(Omentin-1)、25-羟维生素D_(3)[25(OH)D_(3)]水平,HE染色观察肾组织和股骨结构,免疫组化染色检测肾、骨组织JNK、p-JNK蛋白表达,PCR检测肾组织中JNK mRNA水平。结果与模型组比较,壮骨止痛方组和阳性药物组大鼠血清25(OH)D_(3)、Omentin-1水平升高(P<0.01),骨、肾组织JNK、p-JNK蛋白平均灰度值降低(P<0.01),骨、肾组织JNK mRNA水平降低(P<0.01)。结论壮骨止痛胶囊可有效改善绝经后骨质疏松大鼠的骨微结构,其机制可能与调控JNK信号通路有关。
Objective To investigate the effects of Zhuanggu Zhitong Capsules on JNK signaling molecules and their phosphorylated proteins in postmenopausal osteoporosis model female mice.Methods The rats were divided into sham-operation group,blank group,model group,positive drug group,and Zhuanggu Zhitong Capsules group according to the random number table method,with 10 rats in each group.The model group,the positive drug group and the Zhuanggu Zhitong Capsules group were prepared by bilateral ovarian detomy to prepare a female mouse model of postmenopausal osteoporosis.The positive drug group was given 0.9 mg/kg of alendronate sodium,the Zhuanggu Zhitong Capsules group was given was Zhuanggu Zhitong Capsules 1.944 g/kg for gavage,and the blank group,sham-operation group,and model group were given the same volume of normal saline for gavage,once a day for a total of 13 weeks.Rat vaginal exfoliated cells were stained with Wright's staining;serum Omentin-1 and 25(OH)D_(3)levels were determined by ELISA;renal tissue and femoral structure were observed by HE staining;JNK and p-JNK protein expressions were detected by immunohistochemical staining;JNK mRNA levels were detected by PCR.Results Compared with the model group,the serum levels of 25(OH)D_(3)and Omentin-1 in the Zhuanggu Zhitong Capsules group and the positive drug group increased(P<0.01),the mean gray values of JNK and p-JNK protein in bone and kidney tissues decreased(P<0.01),and the mRNA levels of JNK in bone and kidney tissues decreased(P<0.01).Conclusion Zhuanggu Zhitong Capsules can effectively improve the bone microstructure of postmenopausal osteoporotic rats,and its mechanism may be related to the regulation of JNK signaling pathway.
作者
吴美华
李荣慧
俞赟丰
郭冰
张国民
何清湖
雷晓明
夏新斌
Wu Meihua;Li Ronghui;Yu Yunfeng;Guo Bing;Zhang Guomin;He Qinghu;Lei Xiaoming;Xia Xinbin(Graduate School of Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《国际中医中药杂志》
2025年第5期630-637,共8页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81973920、81973863)
湖南省自然科学基金(2021JJ30489)
湖南省教育厅科学研究项目(22A0264)
湖南中医药大学研究生创新课题(2024CX042)
湖南中医药大学2024年“一方”研究生创新项目(2024YF10)。
作者简介
通信作者:夏新斌,Email:280771087@qq.com。
