摘要
目的探讨上皮性卵巢癌(EOC)组织中Smad泛素化调节因子2(SMURF2)、蛋白激酶C受体1(RACK1)的表达及预后价值。方法选取该院2019年1月至2021年1月收治的112例EOC患者(EOC组)的癌组织标本,以及同期在该行卵巢手术的60例卵巢其他疾病患者(对照组)的正常卵巢组织标本。采用实时荧光定量PCR法和免疫组化法检测EOC癌组织及正常卵巢组织SMURF2、RACK1表达。采用Pearson相关分析EOC癌组织中SMURF2 mRNA和RACK1 mRNA表达的关系,Kaplan-Meier生存曲线和Log-rank检验分析不同SMURF2、RACK1表达患者生存预后的差异,多因素COX回归分析EOC预后影响因素。结果EOC癌组织SMURF2 mRNA表达水平低于正常卵巢组织、RACK1 mRNA表达水平高于正常卵巢组织,差异均有统计学意义(P<0.05)。EOC癌组织SMURF2 mRNA与RACK1 mRNA表达水平呈负相关(r=-0.764,P<0.001)。EOC癌组织中SMURF2阳性率低于正常卵巢组织,RACK1阳性率高于正常卵巢组织,差异有统计学意义(P<0.001)。与国际妇产科联盟(FIGO)分期Ⅰ~Ⅱ期、无淋巴结转移EOC患者癌组织相比,FIGO分期Ⅲ期、淋巴结转移EOC患者癌组织中SMURF2阳性率较低、RACK1阳性率较高,差异有统计学意义(P<0.05)。SMURF2阳性组EOC患者3年总生存率高于阴性组、RACK1阳性组EOC患者3年总生存率低于阴性组,差异有统计学意义(Log-rank χ^(2)=4.938、5.251,P=0.028、0.018)。FIGO分期Ⅲ期、淋巴结转移、RACK1阳性是影响EOC生存预后的危险因素(P<0.001),SMURF2阳性是保护因素(P<0.001)。结论EOC癌组织中SMURF2表达水平降低、RACK1表达水平升高,二者与FIGO分期、淋巴结转移有关,是评估EOC患者生存预后的标志物。
Objective To explore the expression and prognostic value of Smad ubiquitination regulator 2(SMURF2)and protein kinase C receptor 1(RACK1)in epithelial ovarian cance r(EOC)tissues.Methods Cancer tissue specimens of 112 patients with EOC(EOC group)admitted to the hospital from January 2019 to January 2021,as well as normal ovarian tissue specimens of 60 patients with other ovarian diseases(control group)who underwent ovarian surgery in the same hospital during the same period were selected.The expressions of SMURF2 and RACK1 in EOC cancer tissues and normal ovarian tissues were detected by real-time fluorescence quantitative PCR and immunohistochemistry.Pearson correlation analysis was used to analyze the relationship between the expressions of SMURF2 mRNA and RACK1 mRNA in EOC cancer tissues.The Kaplan-Meier survival curve and Log-rank test were used to analyze the differences in survival prognosis among patients with different expressions of SMURF2 and RACK1.Multivariate COX regression analysis was conducted to analyze the influencing factors of survival prognosis in EOC.Results The expression level of SMURF2 mRNA in EOC cancer tissues was lower than that in normal ovarian tissues,and the expression level of RACK1 mRNA was higher than that in normal ovarian tissues,and the differences were statistically significant(P<0.05).The expression levels of SMURF2 mRNA and RACK1 mRNA in EOC cancer tissues were negatively correlated(r=-0.764,P<0.001).The positive rate of SMURF2 in EOC cancer tissues was lower than that in normal ovarian tissues,and the positive rate of RACK1 was higher than that in normal ovarian tissues.The difference was statistically significant(P<0.001).Compared with the cancer tissues of EOC patients with International Federation of Obstetrics and Gynecology(FIGO)stage Ⅰ-Ⅱ and no lymph node metastasis,the positive rate of SMURF2 was lower and the positive rate of RACK1 was higher in the cancer tissues of EOC patients with FIGO stageⅢand lymph node metastasis,and the differences were statistically significant(P<0.05).The 3-year overall survival rate of EOC patients in the SMURF2 positive group was higher than that in the negative group,and the 3-year overall survival rate of EOC patients in the RACK1 positive group was lower than that in the negative group,and the differences were statistically significant(Log-Rank χ^(2)=4.938,5.251;P=0.028,0.018).FIGO stageⅢ,lymph node metastasis and positive RACK1 were risk factors affecting the survival prognosis of EOC(P<0.001),while positive SMURF2 was a protective factor(P<0.001).Conclusion The expression level of SMURF2 is decreased and the expression level of RACK1 is increased in EOC cancer tissues.Both are related to FIGO stage and lymph node metastasis,and are markers for evaluating the survival prognosis of EOC patients.
作者
张金辉
李维玲
殷婷
闫璐
ZHANG Jinhui;LI Weiling;YIN Ting;YAN Lu(Department of Obstetrics and Gynecology,Xi′an Gaoxin Hospital,Xi′an,Shaanxi 710075,China)
出处
《国际检验医学杂志》
2025年第12期1425-1430,共6页
International Journal of Laboratory Medicine
基金
陕西省重点研发计划项目(S2022-YF-YBSF-0043)。
作者简介
张金辉,女,主治医师,主要从事妇产科方向临床研究;通信作者:闫璐,E-mail:yl00201683@163.com。
