摘要
目的 观察茵芍二黄汤对小鼠免疫性肝炎的影响,探讨其可能的机制研究。方法 将48只小鼠随机分为模型组、中药高、中、低(53、26.5、13.25 g/kg)剂量组、空白组、西药对照组,连续灌胃4周;每周1次尾静脉注射刀豆蛋白A(ConA)诱导自身免疫性肝炎模型,造模4周,测定肝脾指数,血清ALT、AST水平及IL-10、IL-17A水平,HE染色观察肝组织病理情况,Western blot法检测肝组织Foxp3、RORγt蛋白的表达。结果 与空白组比较,模型组肝脾指数、ALT、AST、IL-17A水平普遍升高,IL-10水平显著降低,肝损伤明显,Foxp3蛋白表达下降,RORγt蛋白表达升高;与模型组比较,中药高、中剂量组和西药对照组肝脾指数、AST、ALT、IL-17A水平均降低,IL-10水平升高,肝损伤程度减轻,Foxp3蛋白表达升高、RORγt蛋白表达降低。结论 茵芍二黄汤对刀豆蛋白引起的小鼠免疫性肝炎有一定保护作用,其机制可能与炎性因子失衡有关。
Objective To observe the effect of Yinshao Erhuang Decoction on immune hepatitis in mice and explore its potential mechanism.Methods A total of 48 mice were randomly divided into a model group,Chinese medicine high-,medium-,and low-dose(53,26.5,13.25 g/kg)groups,a blank group,and a western medicine control group,and they were intervened by gavage continuously for 4 weeks.Concanavalin A(ConA)was injected into the tail vein once a week to induce an autoimmune hepatitis model,with the model constructed after 4 weeks.The liver and spleen indexes,serum ALT and AST levels,and IL-10 and IL-17A levels were measured.HE staining was used to observe the pathological conditions of liver tissue,and Western blot was used to detect the protein expression of Foxp3 and nuclear orphan receptor(RORγt)in liver tissue.Results Compared with the blank group,the liver and spleen indexes,ALT,AST and IL-17A levels were generally increased in the model group,while the IL-10 level was significantly decreased,the liver injury was obvious,and the protein expression of Foxp3 was decreased,while the RORγt protein expression was increased.Compared with the model group,the liver and spleen indexes,AST,ALT and IL-17A levels were decreased in the high-and medium-dose groups and the western control group,while the IL-10 levels were increased,and the liver injury was alleviated,and the Foxp3 protein expression was increased,while the RORγt protein expression was decreased.Conclusion Yinshao Erhuang Decoction has a certain protective effect on immune hepatitis induced by ConA in mice,and its mechanism may be related to regulating the Treg/Th17 imbalance.
作者
景瑞晓
刘全忠
JING Ruixiao;LIU Quanzhong(The First Clinical College of Medicine,Henan University of Chinese Medicine,Zhengzhou 450000,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China)
出处
《吉林中医药》
2025年第6期701-705,共5页
Jilin Journal of Chinese Medicine
基金
河南省中医药科学研究专项课题(2022ZY2013)。
作者简介
景瑞晓(1990-),女,硕士,主治医师,主要从事肝脏疾病临床与实验研究;通信作者:刘全忠,电子信箱-hy2000-514-l@163.com。
