摘要
本研究旨在优化扁蓿豆(Medicago ruthenica)花粉离体萌发的培养条件。通过单因素试验初步筛选出蔗糖、硼酸和氯化钙的最适浓度范围,并利用三因素两水平试验进一步优化培养条件,比较不同处理下花粉萌发率和花粉管生长长度。结果表明,培养基组分、温度及采样时间等因素对花粉萌发有极显著影响。最优培养基组分为蔗糖(200 g·L^(-1))、硼酸(40 mg·L^(-1))和氯化钙(30 mg·L^(-1)),在此条件下,花粉萌发率达75.98%,花粉管长度为224.73μm。最佳培养时间为6 h,最适采样时间为中午12:00—13:00,且在黑暗条件下花粉萌发效果更好,最佳培养条件为25℃,最佳贮藏温度为4℃。本研究结果为扁蓿豆花粉生物学研究及育种实践提供了理论依据。
The aim of this study was to optimize the in vitro germination conditions of Medicago ruthenica pollen.A single-factor experimental design was employed preliminarily to determine the optimal concentration ranges of sucrose,boric acid,and calcium chloride.Subsequently,a three-factor,two-level experimental design was conducted to further optimize the culture conditions,and the pollen germination rates and pollen tube lengths under different treatments were statistically compared.The results demonstrated that medium composi-tion,temperature,and sampling time had highly significant effects on pollen germination.The optimal medium composition was determined to be sucrose(200 g·L^(-1)),boric acid(40 mg·L^(-1)),and calcium chloride(30 mg·L^(-1)).Under these conditions,pollen germination rate reached 75.98%and pollen tube length was 224.73μm.The optimal culture time was 6 h,the optimum sampling time was 12:00-13:00.Pollen culture was more favor-able under dark,the optimal culture temperature was 25℃and the optimal storage temperature was 4℃.The findings of this study provide a theoretical foundation and technical reference for pollen biology research and breeding practices of M.ruthenica.
作者
钟华
向清源
尚晓岚
朱慧森
李钰莹
ZHONG Hua;XIANG Qing-yuan;SHANG Xiao-an;ZHU Hui-sen;LI Yu-ying(College of Grassland Science,Shanxi Agricultural University,Jinzhong,Shanxi Province 030801,China)
出处
《草地学报》
北大核心
2025年第6期1791-1799,共9页
Acta Agrestia Sinica
基金
山西重点研发计划课题(202102140601006-3)
山西省中央引导地方科技发展资金项目(YDZJSX2022B006)资助。
关键词
扁蓿豆
离体萌发
液体培养基
培养条件
Medicago ruthenica
In vitro germination
Liquid medium
Culture conditions
作者简介
钟华(1979-),女,汉族,山西省岢岚县人,博士,实验师,主要从事牧草种质资源开发与利用研究,E-mail:zhonghua109@126.com;通信作者:李钰莹,E-mail:liyuy_ing@163.com。
