摘要
目的 检测5'-tRF-GlyGCC在外周血中的含量,并探讨其含量在结直肠癌(colorectal cancer,CRC)早期诊断中的应用价值。方法 随机收集同一时期贵州医科大学附属医院99例结直肠癌患者、11例肠炎患者、17例结直肠息肉患者和99例健康人群外周血标本,用实时荧光定量PCR检测外周血总RNA中的5'-tRF-GlyGCC,建立受试者工作特征曲线(ROC),分析5'-tRF-GlyGCC含量在结直肠癌早期诊断中的价值,并探讨其含量与CRC患者临床、病理特征之间的关系。结果 5'-tRF-GlyGCC在CRC患者及结直肠息肉患者外周血中的含量显著高于健康人群及肠炎患者,差异有统计学意义(P <0.05)。5'-tRF-GlyGCC诊断结直肠癌的曲线下面积(AUC)为0.817(95%CI:0.763~0.871),诊断灵敏度为56.7%,特异度为99.9%。外周血中5'-t RF-GlyGCC含量与CRC患者CEA、CA19-9水平显著正相关。结论 检测外周血中5'-tRFGlyGCC在CRC早期诊断中具有较高应用价值。
Objective To detect the content of 5'-tRF-GlyGCC in peripheral blood and explore its applica‐tion value in the early diagnosis of colorectal cancer(CRC).Methods Peripheral blood samples were randomly collected from 99 CRC patients,11 patients with enteritis,17 patients with colorectal polyps and 99 healthy indi‐viduals in the same period at the Affiliated Hospital of Guizhou Medical University.The content of 5'-tRF-GlyGCC in total RNA of peripheral blood was detected by fluorescence RT-PCR.The receiver operating characteristic curve(ROC)was established to analyze the value of 5'-tRF-GlyGCC content in the early diagnosis of CRC and to explore the relationship between its content and the clinical and pathological characteristics of CRC patients.Results The content of 5'-tRF-GlyGCC in the peripheral blood of CRC patients and colorectal polyp patients was significantly higher than that of healthy individuals and patients with enteritis,and the differences were statistically significant(P<0.05).The area under the curve(AUC)of 5'-tRF-GlyGCC for diagnosing CRC was 0.817(95%CI:0.763~0.871),with a diagnostic sensitivity of 56.7%and a specificity of 99.9%.The content of 5'-tRF-GlyGCC in peripheral blood was significantly positively correlated with the levels of CEA and CA19-9 in CRC patients.Conclusion The detection of 5'-tRF-GlyGCC in peripheral blood has a high application value in the early diagnosis of CRC and can be used for clinical reference and research.
作者
王龙
吴映敏
尚莎
王璐
WANG Long;WU Yingmin;SHANG Sha;WANG Lu(Department of Physiology,School of Basic Medical Science,Guizhou Medical University,Guiyang 561113,Guizhou,China;不详)
出处
《实用医学杂志》
北大核心
2025年第11期1730-1735,共6页
The Journal of Practical Medicine
基金
国家自然科学基金地区基金项目(编号:82260606)
国家自然科学基金青年基金项目(编号:82103296)
贵州省科技厅科技计划项目(编号:黔科合基础-ZK[2022]一般390)
贵州省教育厅青年科技人才成长项目(编号:黔教技[2022]191)
中国博士后科学基金面上二等资助项目(编号:2021M700971)
贵州医科大学优秀青年人才项目青年拔尖基金项目(编号:贵医大优秀青年人才(2022)109)
贵州医科大学国家自然科学基金培育项目(编号:20NSP009)
贵州省卫生健康委基金科学技术基金项目(编号:gzwkj2022-254)。
作者简介
通信作者:王璐,E-mail:2899374290@qq.com。
