摘要
该试验通过血浆代谢组以及酶联免疫分析探究驼乳对小鼠抗炎及免疫功能的影响。将32只ICR小鼠随机分成驼奶组与对照组2组,每天分别灌喂10 mL/kg体重驼奶与纯净水,灌喂周期28 d。灌喂结束后,采集肝脏与血液样本,通过酶联免疫法测定小鼠炎症指标,采用液质联用对其血浆代谢物进行非靶向代谢组学检测,并对检测结果进行分析。结果显示,灌喂驼奶能提高小鼠肝脏中白细胞介素6(interleukin 6,IL-6)、分泌型免疫球蛋白A(secretory immunoglobulin A,sIgA)、白介素-1β(interleukin 1β,IL-1β)和干扰素γ(interferonγ,IFN-γ)水平、降低肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)水平;2组小鼠中共筛选出207个差异代谢物,其中对照组有201个差异代谢物显著上调,有6个差异代谢物显著下调,这207个差异代谢物中溶血磷脂酰胆碱等脂质代谢物占比最多,这些差异代谢物显著富集到嘧啶代谢、三羧酸循环等通路中。该研究结果表明,驼乳可能通过对脂代谢及嘧啶代谢和三羧酸循环等通路调控从而达到对小鼠抗炎作用进行调节,研究结果可为驼乳的功能性开发与利用提供数据参考。
This study investigated the effects of camel milk on the anti-inflammatory and immune functions of mice through plasma metabolomics and enzyme-linked immunosorbent assay(ELISA)analysis.A total of 32 ICR mice were randomly divided into two groups,including a camel milk group and a control group.The mice were orally administered 10 mL/kg body weight of camel milk or distilled water daily for 28 days.After the administration period,liver and blood samples were collected.Inflammatory markers in the mice were measured using ELISA,and non-targeted metabolomics of serum metabolites was conducted using liquid chromatography-tandem mass spectrometry(LC-MS/MS),followed by analysis of the results.Results demonstrated that camel milk administration elevated the levels of interleukin 6(IL-6),secretory immunoglobulin A(sIgA),interleukin 1β(IL-1β),and interferonγ(IFN-γ),while reducing tumor necrosis factor-α(TNF-α)levels in the liver of mice.A total of 207 differential metabolites were identified between the two groups,with 201 metabolites significantly upregulated and 6 significantly downregulated in the control group.Among these differential metabolites,lipid metabolites such as lysophosphatidylcholine(LPC)accounted for the majority,and these metabolites were significantly enriched in pathways including pyrimidine metabolism and the tricarboxylic acid(TCA)cycle.These findings suggest that camel milk may exert its anti-inflammatory effects in mice by modulating lipid metabolism,pyrimidine metabolism,and the TCA cycle.The results provide valuable data for the functional development and utilization of camel milk.
作者
刘师博
姚新奎
孟军
曾亚琦
孙志文
王建文
LIU Shibo;YAO Xinkui;MENG Jun;ZENG Yaqi;SUN Zhiwen;WANG Jianwen(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Key Laboratory of Horse Breeding and Exercise Physiology,Urumqi 830052,China)
出处
《食品与发酵工业》
北大核心
2025年第12期123-131,共9页
Food and Fermentation Industries
基金
中央引导地方科技发展专项资金项目(ZYYD2023C02-2)
自治区创新环境(人才、基地)建设专项(PT2311)
自治区重大科技专项(2022A02013-1)。
关键词
驼乳
代谢组学
抗炎作用
小鼠
camel milk
metabolomics
anti-inflammatory
mice
作者简介
第一作者:刘师博,硕士研究生;通信作者:王建文,副教授,E-mail:275139618@qq.com。
