摘要
目的:优化肾安汤的提取工艺,分析其化学成分。方法:以毛蕊异黄酮葡萄糖苷、丹酚酸B的含量及浸膏得率作为综合评分的指标,将提取次数、提取时间、加水量作为考察因素,采用Box-Behnken响应面法,在单因素试验基础上优选肾安汤的提取工艺并开展验证试验;利用超高效液相色谱-四级杆飞行时间串联质谱(UPLC-Q-TOF-MS)技术分析肾安汤的化学成分。结果:肾安汤最佳提取工艺为提取次数3次,提取时间60 min,加水量12倍。肾安汤中鉴定出了74种化学成分,主要包括黄酮类、皂苷类和有机酸类等,并对这些化合物的来源进行了归属。结论:优化后的肾安汤提取工艺稳定可行、重现性好,建立的UPLC-Q-TOF-MS分析方法可对肾安汤中的化学成分进行快速识别和鉴定,可为肾安汤药效物质基础和质量控制的研究提供科学依据。
Objective:To optimize the extraction process of Shen'an decoction and analyze its chemical components.Methods:The content of calycosinglucoside,salvianolic acid B and extract yields were taken as the comprehensive scoring indicators,and the extraction frequency,extraction time,and amount of water added were taken as factors.Based on the single factor test,the extraction was optimized by Box-Behnken design-response surface methodology,and validation tests were carried out.Tostudy the chemical constituents in Shenan decoction by(ultra performance liquid chromatography-quadrupole time of flight high resolution-mass spectrometry,UPLC-Q-TOF-MS).Results:The optimized extraction process of Shen'an decoction included 3 times of extractions,60 min of extraction and 12 times of water.A total of 74 chemical constituents in Shen'an decoction were identified,including flavonoids,saponins and acids,and the medicinal sources of the compounds were assigned.Conclusion:The extraction process of Shen'an decoction is stable,feasible and reproducible after optimization.The established method of UPLC-Q-TOF-MS can quickly identify the chemical components in Shen'an decoction,and provide scientific basis for the study of the pharmacodynamic substance basis and quality control of Shen'an decoction.
作者
杨梅萍
谢瑜
王艾琳
邓钰文
匡创富
刘杨
YANG Meiping;XIE Yu;WANG Ailin;DENG Yuwen;KUANG Chuangfu;LIU Yang(The Second Hospital Affiliated of Hunan University of Chinese Medicine,Changsha Hunan 410005,China)
出处
《中医药导报》
2025年第5期63-70,共8页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省卫生健康委卫生科研项目(W20243268)
湖南中医药大学校院联合基金项目(2024XYLH052)。
作者简介
通信作者:谢瑜,男,主管药师,研究方向为中医药防治代谢性疾病作用机理与物质基础研究。
