摘要
为探究发酵乳杆菌S1荚膜多糖(capsular polysaccharides,CPS)的免疫调节及抗衰老活性,本研究首先测定CPS浓度对RAW264.7细胞的活力、吞噬活性、酸性磷酸酶活性、NO和细胞因子分泌水平的影响并评估其免疫调节活性。再以秀丽隐杆线虫为模型,测定线虫寿命、氧/热应激能力、生殖能力,来探究CPS的抗衰老作用。结果表明,25~200μg/mL范围内CPS能提高RAW264.7细胞的增殖能力、细胞吞噬率和酸性磷酸酶活力,并通过上调相关基因表达促进其NO及细胞因子IL-6、IL-1β和TNF-α的释放(P<0.05)。与对照相比,CPS处理组细胞活力、吞噬率和酸性磷酸酶活力最大提升幅度分别达到58.22%、33.48%和39.5%,NO、IL-6、IL-1β和TNF-α的最大分泌水平分别增加101.29%、31.33%、161.08%和79.90%,从而表现出较好的免疫调节活性。线虫试验表明,50μg/mL的CPS对正常、氧应激和热应激环境下线虫寿命的延长率分别为19.87%、39.39%和29.02%,显示出良好的抗衰老活性。同时,CPS有效提高了线虫的生殖能力,其最高产卵数目较对照组增加了0.19倍。RTqPCR分析揭示CPS是通过协同调控线虫胰岛素信号通路(Daf-16和Sir-2.1)和热应激信号通路(Hsf-1和Hsp-16.2)发挥抗衰老作用的。以上结果表明CPS具有良好的免疫调节活性和抗衰老活性。本研究可以为乳酸菌CPS在免疫调节和抗衰老产品的应用提供理论支撑。
To investigate the immunomodulatory and anti-aging activities of capsular polysaccharides(CPS)from Lactobacillus fermentum S1,this study first determined the effects of CPS concentration on the viability,phagocytic activity,acid phosphatase activity,NO production,and cytokine secretion levels in RAW264.7 cells,and evaluated its immunomodulatory activity.Subsequently,using Caenorhabditis elegans as a model,the effects of CPS on C.elegans lifespan,oxidative/heat stress resistance,and reproductive capacity were assessed to explore its anti-aging properties.The results indicated that within the range of 25~200μg/mL,CPS significantly enhanced cell proliferation ability,phagocytosis rate,and acid phosphatase activity in RAW264.7 cells,and promoted the release of NO and cytokines IL-6,IL-1β,and TNF-αby up-regulating the expression of related genes(P<0.05).Compared to control group,the maximum increase in cell viability,phagocytosis rate,and acid phosphatase activity in the CPS-treated group reached 58.22%,33.48%,and 39.5%,respectively.The maximum secretion levels of NO,IL-6,IL-1β,and TNF-αincreased by 101.29%,31.33%,161.08%,and 79.90%,respectively,demonstrating good immunomodulatory activity.The C.elegans experiment showed that treatment with 50μg/mL CPS extended lifespan of C.elegans under normal,oxygen-stressed,and heat-stressed conditions by 19.87%,39.39%,and 29.02%,respectively,suggesting strong anti-aging activity.CPS also effectively improved the RT-qPCR analysis revealed that CPS exerted its anti-aging effect through co-regulation of insulin signaling pathway(Daf-16 and Sir-2.1)and heat shock signaling pathway(Hsf-l and Hsp-16.2)in C.elegans.These findings indicate that CPS possesses good immunomodulatory and anti-aging activities.This study provides theoretical support for the application of CPS produced by lactic acid bacteria in immunoregulation and anti-aging products.
作者
王坤
邰孟蝶
潘禹溪
李思露
李梦迪
赵婧
左锋
WANG Kun;TAI Mengdie;PAN Yuxi;LI Silu;LI Mengdi;ZHAO Jing;ZUO Feng(College of Food Science,Heilongjiang Bayi Agricultural Univercity,Daqing 163319,China;National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural Univercity,Daqing 163319,China;Engineering Research Center for Processing and Utilization of Grain By-products,Ministry of Education,Daqing 163319,China)
出处
《食品工业科技》
北大核心
2025年第12期360-369,共10页
Science and Technology of Food Industry
基金
黑龙江省自然科学基金优秀青年项目(YQ2021C029)。
关键词
乳酸菌
荚膜多糖
免疫调节
抗衰老
lactic acid bacteria
capsular polysaccharide
immune regulation
anti-aging
作者简介
王坤(1987-),男,博士,副教授,研究方向:食品微生物与生物技术,E-mail:wangkun6zzu@126.com;通信作者:左锋(1979-),男,博士,教授,研究方向:植物蛋白及农副产品加工利用,E-mail:zuofeng-518@126.com。
