摘要
为明确青藏高原不同地理位点采集的中华羊茅(Festuca sinensis)种带真菌的种类和分布,并了解内生真菌感染率是否影响其多样性,本研究选取从青海、西藏等不同地点同一生长季采集的11份中华羊茅种质资源,对种带真菌进行分离纯化,通过传统形态学和分子生物学进行鉴定,并分析种子采集地环境因子对真菌多样性指数的影响。结果表明:1)从11份中华羊茅种质资源中共分离得到199株真菌,经过初步形态学分类为92种真菌,其中对50种真菌进行了鉴定,共鉴定出20属14种,种带真菌主要由枝孢属(Cladosporium)、镰刀菌属(Fusarium)、亚隔孢壳属(Didymella)、毛座球腔菌属(Trichometasphaeria)和异壳二孢属(Neoascochyta)组成,其中枝孢属为优势属。2)不同种质间的真菌菌种分布和真菌多样性存在一定的差异;冗余分析表明,生长季月平均降水量是推动种带真菌群落变化的主要环境因素,而内生真菌感染率对中华羊茅种带真菌多样性无显著影响(P>0.05)。综上可知,中华羊茅种带真菌具有多样性,其受生长季月平均降水量影响,与内生真菌感染率无关。
In this study,11 germplasm resources of Festuca sinensis were collected from different areas of the Qinghai-Tibet Plateau during the same growing season.The purpose was to identify the species and distribution of seed-borne fungi in Festuca sinensis collected from different geographic locations on the Qinghai-Tibet Plateau and to understand whether the infection rate of endophytic fungi affects their diversity.The fungal strains were isolated,purified,and identified using traditional morphological and molecular biological methods.The effects of environmental factors from the seed collection sites on fungal diversity index were analyzed.The results showed that:1)A total of 92 culturable fungal strains were isolated from 11 F.sinensis germplasm resources,which were preliminarily classified into 92 fungal species based on morphology.Among these,50 fungal species were identified,belonging to 20 genera and 14 species.The fungal community mainly consisted of Cladosporium,Fusarium,Didymella,Trichometasphaeria,and Neoascochyta,with Cladosporium being the most abundant genus.2)There were major differences in fungal species distribution and diversity across different germplasm resources.Redundancy analysis showed that the growing monthly mean precipitation was the primary environmental factor driving changes in the seed-borne fungal community,while the endophytic fungal infection rate had no significant effect on the diversity of seed-borne fungi in F.sinensis(P>0.05).Overall,the findings revealed that the seed-borne fungi of F.sinensis exhibit diversity.Their distribution and community structure were influenced by the growing monthly mean precipitation,with no significant correlation with the endophytic fungal infection rate.
作者
普玥
田沛
吴涵
PU Yue;TIAN Pei;WU Han(College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730000,Gansu,China)
出处
《草业科学》
北大核心
2025年第4期1013-1023,共11页
Pratacultural Science
基金
甘肃省交通运输厅科研项目(2021-28)。
关键词
中华羊茅
真菌鉴定
内生真菌
种带真菌多样性
环境因子
优势属
种质资源
Festuca sinensis
fungal identification
Epichloësinensis
seed-borne fungal diversity
environmental factors
abundant genus
germplasm resources
作者简介
第一作者:普玥(2002-),女,云南昆明人,在读本科生,研究方向为草业微生物。E-mail:puy21@lzu.edu.cn;通信作者:田沛(1979-),女,河南新郑人,教授,博士,研究方向为草业微生物。E-mail:tianp@lzu.edu.cn。
