摘要
水环境中致病微生物的快速精准检测是保证生态环境安全和人类健康的重要保障。水环境中潜在致病微生物种类繁多,通常含量较低但风险极大,这对检测方法的通量和灵敏度提出了要求。同时,生物风险应急响应也对致病微生物的便携和快速检测提出了要求。CRISPR/Cas检测技术具有高效性、特异性和易操作性,在致病微生物核酸快检领域体现出巨大潜力。从检测灵敏度、多重检测、对象适配性和便携装置4个方面出发,系统综述了国际上有关CRISPR/Cas在致病微生物核酸检测领域的最新研究进展。此外,结合水环境监测领域的实际特点,指出了CRISPR/Cas致病微生物快检技术面临的挑战,并提出了相应的建议。本文将为CRISPR/Cas在水环境中致病微生物快检中的推广应用和方法构建提供指导。
The rapid and precise detection of pathogenic microorganisms in aquatic environments is crucial for ecological security and human health.Due to the vast diversity of potential pathogenic microorganisms,their typically low concentrations but high risks impose stringent requirements of throughput and sensitivity of detection methods.Additionally,emergency responses to biological risks necessitate the capability to be portable and deliver rapid results.The CRISPR/Cas system,known for its efficiency,specificity,and ease of operation,has demonstrated significant potential in the rapid nucleic acid detection of pathogenic microorganisms.This paper reviews recent internationally renowned advancements in CRISPR/Cas-based rapid detection of pathogenic microorganisms,drawing insights from 4 aspects of detection sensitivity,multiplexed detection,adaptability to different nucleic acid targets,and portable devices.Furthermore,considering the specific characteristics of water environment monitoring,this paper highlights the challenges faced by CRISPR/Cas-based rapid detection technology for pathogenic microorganisms and provides corresponding recommendations.This review aims to guide the development and application of CRISPR/Cas technology for the rapid detection of pathogenic microorganisms in water environments.
作者
何文祥
胡丹心
蒙启宇
李敦柱
张远
李波
HE Wenxiang;HU Danxin;MENG Qiyu;LI Dunzhu;ZHANG Yuan;LI Bo(Guangzhou Ecological Environment Monitoring Center Station,Guangzhou Guangdong 510006;Guangdong Basic Research Center of Excellence for Ecological Security and Green Development,School of Ecology,Environment and Resources,Guangdong University of Technology,Guangzhou Guangdong 510006;Jiyang College of Zhejiang A&F University,Shaoxing Zhejiang 311800;College of Environment and Resources,Zhejiang A&F University,Hangzhou Zhejiang 311300)
出处
《环境污染与防治》
北大核心
2025年第5期41-44,I0003,共5页
Environmental Pollution & Control
基金
国家重点研发计划项目(No.2021YFC3201004)
广东省引进创新创业团队计划项目(No.2021ZT090543)。
作者简介
第一作者:何文祥,男,1990年生,硕士,高级工程师,主要从事水生态监测相关研究;通信作者:李波。
