摘要
【目的】对火龙果成花素FT基因(SmFT)进行克隆及时空表达模式分析,为人工补光诱导火龙果成花及选育光钝感型火龙果新品种提供理论依据。【方法】以金都1号红肉火龙果为研究对象,克隆SmFT基因并对其进行生物信息学分析;采用实时荧光定量PCR检测SmFT基因在火龙果不同组织中及不同波长补光诱导下的表达模式。【结果】SmFT基因的开放阅读框(ORF)为528 bp,编码175个氨基酸残基。生物信息学分析结果显示,SmFT蛋白为不稳定的亲水性蛋白,呈碱性,属于非分泌型蛋白和非膜蛋白;该蛋白定位于细胞质、叶绿体和细胞核的概率较高。SmFT蛋白含有保守的PEBP结构域,与华东葡萄、小果葡萄和满天星的同源蛋白亲缘关系较近,而与红叶藜、小藜和苋菜同源蛋白的亲缘关系较远。实时荧光定量PCR检测结果显示,SmFT基因具有明显的组织表达特异性,在火龙果花萼中的相对表达量显著高于其他组织(P<0.05),是幼嫩花蕾中的15.25倍,是幼嫩花蕾邻近茎组织的2.75倍,是叶芽的2.24倍;SmFT基因的相对表达量在火龙果幼嫩花蕾邻近茎组织和叶芽中较高,在成熟果皮、成熟果肉、根和成熟茎等组织中较低,在成熟茎中几乎不表达。随着补光日期的延长,SmFT基因的相对表达量在不同波长的光质诱导下均呈先升高后降低的变化趋势,且均在光诱导43 d后达峰值,在光诱导50 d后(现蕾期)急剧下降,SmFT基因在波长660 nm红光诱导下相对表达量最高,其次是630 nm红光和590 nm黄光,最低是450 nm蓝光。【结论】SmFT基因具有明显的组织表达特异性和不同光质诱导表达差异性,推测该基因可能参与火龙果花芽的前期组织分化诱导和后期花器官或茎的发育调控,在火龙果成花转变过程中发挥重要作用。
【Objective】Cloning and spatiotemporal expression pattern analysis of the FT gene(SmFT)of pitaya flower formation were conducted to provide theoretical basis for artificial supplementary light induction of pitaya flower formation and breeding of new light-insensitive pitaya varieties.【Method】The red-fleshed pitaya Jindu 1 was selected as the research object to clone the SmFT gene,which was then performed bioinformatics analysis on.The expression patterns of the SmFT gene in different tissues of pitaya and under the induction of supplementary light at different wave‐lengths were detected by real-time fluorescence quantitative PCR.【Result】The open reading frame(ORF)of SmFT gene was 528 bp and encoded 175 amino acid residues.Bioinformatics analysis showed that SmFT protein was an unstable hydrophilic protein with alkalinity,belonging to non-secretory protein and non-membrane protein.Subcellular localiza‐tion predicted that SmFT protein was highly likely to be localized in cytoplasm,chloroplast and nucleus.SmFT protein contained a conserved PEBP domain and was closely related to homologous proteins of Vitis vinifera,Vitis serrata and Gypsophila paniculata,while distantly related to homologous proteins of Oxybasis rubra,Chenopodium ficifolium and Amaranthus tricolor.Real-time fluorescence quantitative PCR analysis showed that SmFT gene had obvious tissue expres‐sion specificity.The relative expression level of SmFT gene in pitaya calyx was significantly higher than that in other tis‐sues,and was as 15.25 times as that in tender floral buds,2.75 times as that in stem tissues adjacent to tender floral buds(P<0.05),and 2.24 times as that in stem buds.The relative expression level of SmFT gene was higher in the stems and stem buds near the tender floral buds of pitaya,while lower in the mature peel,mature flesh,roots and mature stems,and almost not expressed in the mature stems.With the extension of supplementary lighting days,the relative expression of SmFT gene showed a trend of increasing first and then decreasing under the induction of light quality of different wave‐lengths,and reached the peak value after 43 d of light induction,and dropped sharply after 50 d(bud stage).The relative expression level of SmFT gene was the highest under the induction of red light with a wavelength of 660 nm,followed by red light of 630 nm and yellow light of 590 nm,and was the lowest under blue light of 450 nm.【Conclusion】SmFT gene has obvious tissue expression specificity and expression differences induced by light of various wavelengths.It is specu‐lated that SmFT may be involved in the early floral bud differentiation induction and the later developmental regulation of floral organs or stems,and play an important role in the floral initiation process of pitaya.
作者
武志江
黄泽键
叶小滢
邓海燕
陆贵锋
梁桂东
韦朔曈
刘朝安
李祯英
黄黎芳
WU Zhi-jiang;HUANG Ze-jian;YE Xiao-ying;DENG Hai-yan;LU Gui-feng;LIANG Gui-dong;WEI Shuo-tong;LIU Chao-an;LI Zhen-ying;HUANG Li-fang(Horticultural Research Institute,Guangxi Academy of Agricultural Sciences/Nanning Investigation Station of South Sub‐tropical Fruit Trees,Ministry of Agriculture and Rural Affairs,Nanning,Guangxi 530007,China)
出处
《南方农业学报》
北大核心
2025年第4期1193-1202,共10页
Journal of Southern Agriculture
基金
国家自然科学基金项目(32202464)
广西自然科学基金项目(2023GXNSFBA026055)
广西农业科学院基本科研业务专项(桂农科2025YP042,桂农科2025YP046)。
作者简介
第一作者:武志江(1988-),https://orcid.org/0000-0002-0985-2408,副研究员,主要从事火龙果优异基因挖掘与分子育种研究工作,E-mail:zhijiangwu@gxaas.net;通信作者:黄黎芳(1984-),https://orcid.org/0000-0002-2482-2207,副研究员,主要从事火龙果遗传育种与栽培研究工作,E-mail:huanglifang@gxaas.net。
