摘要
为提高板栗花黄酮高值化利用,以酶辅助超声法优化板栗花黄酮提取工艺,并筛选大孔树脂对其纯化,采用单因素试验和响应面试验确定最佳提取条件,同时对纯化前后板栗花黄酮抗氧化活性进行评价。结果表明,最佳提取工艺为料液比1:30(g/mL)、乙醇体积分数61%、纤维素酶用量2.5%、超声温度55℃、超声功率110 W、超声时间50 min,提取率达到4.049%,纯度为5.94%。筛选出大孔树脂AB-8对其纯化效果最佳,在上样液浓度1.5 mg/mL、pH5、上样量30 mL、上样流速1.5 mL/min、吸附平衡后以80%乙醇为洗脱液、用量120 mL、洗脱速率2 mL/min为最佳纯化条件,纯化后纯度提高至29.17%。板栗花黄酮纯化前后对2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基的IC_(50)值分别为41.279、36.284μg/mL,对1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基的IC_(50)值分别为80.661、51.885μg/mL。板栗花通过酶辅助超声法提取,大孔树脂纯化后其黄酮纯度有了明显的提高,纯化前后的板栗花黄酮均具有良好的抗氧化活性,且纯化后抗氧化能力有显著提高,为板栗花的精深开发提供了可行性参考。
In order to improve the high-value utilization of chestnut flower flavonoids,the enzyme assisted ultrasound method was used to optimize the extraction process of chestnut flower flavonoids,and macroporous resins were screened for purification.Single factor experiments and response surface test were used to determine the optimal extraction conditions,and the antioxidant activity of chestnut flower flavonoids before and after purification was evaluated.The results showed that the optimum extraction parameters were material liquid ratio 1:30(g/mL),ethanol concentration 61%,cellulase dosage 2.5%,ultrasound temperature 55℃,ultrasound power 110 W,ultrasound time 50 minutes,and the extraction rate reached 4.049%,the purity rate reached 5.94%.AB-8 macroporous resin had the best effect on the purification of flavonoids from chestnut flowers.The best purification condition was that 1.5 mg/mL loading liquid concentration,pH 5,30 mL loading liquid quantity,1.5 mL/min loading liquid flow rate,80%ethanol as eluent after adsorption equilibrium,120 mL eluent dosage and 2 mL/min flow rate,the purity rate reached 29.17%.The antioxidant activity test showed that the IC_(50) of flavonoids from chestnut flowers for before and after purification scavenging 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)cation radical were 41.279μg/mL,36.284μg/mL,respectively.The IC_(50) of flavonoids from chestnut flowers for before and after purification scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical were 80.661μg/mL,51.885μg/mL,respectively.The chestnut flowers flavonoids was extracted,by enzymeassisted ultrasound method and purification by macroporous resin significantly improved the purity of its flavonoids.Both the pre-purification and post-purification flavonoids of chestnut flowers exhibited good antioxidant activity,with a significantly enhanced antioxidant capacity after purification,providing feasibility references for the in-depth development of chestnut flowers.
作者
陈亚蓝
陈顺心
朱静
李碧含
栗荣昌
侯贺丽
刘开华
李建芳
CHEN Yalan;CHEN Shunxin;ZHU Jing;LI Bihan;LI Rongchang;HOU Heli;LIU Kaihua;LI Jianfang(College of Food Science and Engineering,Xingyang Agriculture and Forestry University,Xinyang 464000,China;Dabie Mountain Characteristic Food Resources Comprehensive Utilization Engineering Technology Research Center,Xinyang 464000,China)
出处
《食品科技》
北大核心
2025年第3期207-216,共10页
Food Science and Technology
基金
河南省重点研发与推广专项(科技攻关)项目(242102110090)
河南省高校研究性教学改革与实践研究项目(2022SYJXLX119)
2023河南省重点研发专项(231111312800)
河南省高等学校重点科研项目(24B550017)
信阳农林学院科技创新团队项目(XNKJTD-001)
信阳农林学院2019年度学校青年基金项目(2019LG009)
信阳农林学院2021年度学校青年基金项目(QN2021028)。
关键词
板栗花
黄酮
酶辅助超声法
提纯工艺
响应面法
抗氧化
chestnut flowers
flavonoids
enzyme-assisted ultrasound method
purification process
response surface methodology
antioxidant
作者简介
陈亚蓝(1991-),女,硕士,讲师,研究方向为天然活性产物开发及食品加工;通信作者:朱静。
